Effects of Astragalus polysaccharides (APS) on the expression of immune response genes in head kidney, gill and spleen of the common carp, Cyprinus carpio L
For fish immune defences, cytokines and anti-microbial peptides (lysozyme) in circulating system play important roles. In the present study, the effects of Astragalus polysaccharides (APS) injection on gene expression of interleukin 1β (IL-1β), tumor necrosis factorα (TNF-α) and lysozyme-C in the he...
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Published in | International immunopharmacology Vol. 8; no. 1; pp. 51 - 58 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
01.01.2008
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | For fish immune defences, cytokines and anti-microbial peptides (lysozyme) in circulating system play important roles. In the present study, the effects of Astragalus polysaccharides (APS) injection on gene expression of interleukin 1β (IL-1β), tumor necrosis factorα (TNF-α) and lysozyme-C in the head kidney, gill and spleen of common carp were determined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). After injection of APS, IL-1β mRNA level increased in a dose-dependent manner in the head kidney, while no significant changes were found in the gill and spleen. High dose of APS up regulated TNF-α transcription in the gill and spleen, while TNF-α mRNA level decreased significantly in the head kidney of low dose of APS. Lysozyme-C mRNA levels were up regulated in the gill of low dose of APS and spleen of middle dose of APS. No effect of the APS on lysozyme-C expression was observed in head kidney. These results constitute a first step toward the understanding of APS effect on cytokines and immune-related gene expression in different organs of common carp. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1567-5769 1878-1705 |
DOI: | 10.1016/j.intimp.2007.10.009 |