Nucleotide sequence analysis of nucleocapsid protein gene of canine distemper virus isolates in Thailand

• Published in: Veterinary microbiology Vol. 105; no. 2; pp. 137 - 142
• Main Authors: Keawcharoen, J., Theamboonlers, A., Jantaradsamee, P., Rungsipipat, A., Poovorawan, Y., Oraveerakul, K.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 31.01.2005; Elsevier Science
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Canine distemper virus; Cluster Analysis; Distemper - virology; Distemper Virus, Canine - genetics; Distemper Virus, Canine - isolation & purification; DNA, Viral - chemistry; DNA, Viral - genetics; Dog Diseases - virology; Dogs; Female; Fundamental and applied biological sciences. Psychology; Male; Microbiology; Miscellaneous; Molecular Sequence Data; Nucleocapsid protein gene; Nucleocapsid Proteins - chemistry; Nucleocapsid Proteins - genetics; Nucleotide sequence; Phylogeny; Polymerase Chain Reaction - veterinary; Sequence Analysis, DNA; Thailand; Viral Vaccines - genetics; Virology; Virulence; Thailand; Asia; Nucleocapsid protein gene; Nucleotide sequence; Canine distemper virus; Dogs; Fissipedia; Carnivora; Microbiology; Nucleocapsid; Protein; Paramyxoviridae; Virus; Paramyxovirinae; Mononegavirales; Vertebrata; Mammalia; Gene; Morbillivirus; Isolate; Veterinary; Dog; Aminoacid sequence
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2004.10.011

The C-terminal part of the nucleocapsid protein gene of 13 canine distemper virus (CDV) isolates from Thailand, were analyzed. The nucleotide sequences were assigned to two clusters; cluster A exhibited a high degree of homology with the vaccine strain Onderstepoort, 99.10 and 97.61%, respectively, in the two isolates examined. Cluster B appeared closely related to virulent strains registered in the GeneBank database and to the virulent reference strain (A75/17); a total of 11 samples were analyzed, with 94.63–99.10% homology at the same position. The deduced amino acid sequences correlated with the two-nucleotide sequence clusters. However, there was no association among the CDV groups with histories of vaccination, sex, ages, clinical findings and evidence of viral antigen in tissues.