Differential functional effects of biomaterials on dendritic cell maturation

• Published in: Acta biomaterialia Vol. 8; no. 10; pp. 3606 - 3617
• Main Authors: Park, Jaehyung, Babensee, Julia E.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2012
• Subjects: agarose; Apoptosis - drug effects; Biocompatibility; biocompatible materials; Biocompatible Materials - pharmacology; Biomaterials; Cell Adhesion - drug effects; Cell Differentiation - drug effects; Cell Movement - drug effects; Cell Proliferation - drug effects; Cell Shape - drug effects; chitosan; Chitosan - pharmacology; Combination products; crosslinking; cytokines; Cytokines - metabolism; Dendritic cells; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - metabolism; Endocytosis - drug effects; Flow Cytometry; Fluorescence; Histocompatibility Antigens Class II - metabolism; Humans; hyaluronic acid; Hyaluronic Acid - pharmacology; immune response; Immunomodulation; Inflammation Mediators - metabolism; Lactic Acid - pharmacology; Lipopolysaccharides - pharmacology; molecular weight; monocytes; phenotype; Photoelectron Spectroscopy; Polyglycolic Acid - pharmacology; Receptors, Cell Surface - metabolism; selection criteria; Sepharose - pharmacology; Surface Properties - drug effects; T-Lymphocytes - cytology; T-Lymphocytes - drug effects; Transcription Factor RelA - metabolism; Biocompatibility; Dendritic cells; Immunomodulation; Combination products; Biomaterials
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2012.06.006

The immunological outcome of dendritic cell (DC) treatment with different biomaterials was assessed to demonstrate the range of DC phenotypes induced by biomaterials commonly used in combination products. Immature DCs (iDCs) were derived from human peripheral blood monocytes, and treated with different biomaterial films of alginate, agarose, chitosan, hyaluronic acid (HA), or 75:25 poly(lactic-co-glycolic acid) (PLGA) and a comprehensive battery of phenotypic functional outcomes was assessed. Different levels of functional changes in DC phenotype were observed depending on the type of biomaterial films used to treat the DCs. Treatment of DCs with PLGA or chitosan films supported DC maturation, with higher levels of DC allostimulatory capacity, pro-inflammatory cytokine release, and expression of CD80, CD86, CD83, HLA-DQ and CD44 compared with iDCs, and lower endocytic ability compared with iDCs. Alginate film induced pro-inflammatory cytokine release from DCs at levels higher than from iDCs. Dendritic cells treated with HA film expressed lower levels of CD40, CD80, CD86 and HLA-DR compared with iDCs. They also exhibited lower endocytic ability and CD44 expression than iDCs, possibly due to an insolubilized (cross-linked) form of high molecular weight HA. Interestingly, treatment of DCs with agarose film maintained the DC functional phenotype at levels similar to iDCs except for CD44 expression, which was lower than that of iDCs. Taken together, these results can provide selection criteria for biomaterials to be used in immunomodulating applications and can inform potential outcomes of biomaterials within combination products on associated immune responses as desired by the application.