Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma
Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of ce...
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| Published in | Biomolecules & biomedicine Vol. 13; no. 3; pp. 186 - 191 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Bosnia and Herzegovina
Association of Basic Medical Sciences
01.08.2013
Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| Subjects | |
| Online Access | Get full text |
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| Abstract | Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan's test at a significance level of P≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma. |
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| AbstractList | Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan's test at a significance level of P<0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose-and time-dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma. Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan’s test at a significance level of P ≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma. Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan's test at a significance level of P<0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose-and time-dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma. KEY WORDS: cytotoxicity, apoptosis, Anticancer, oral squamous cell carcinoma, grape seed extract Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan’s test at a significance level of P≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma. Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan's test at a significance level of P≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma. |
| Audience | Academic |
| Author | Hosseini, Sepideh Vosough Orangi, Mona Gharavi, Nader Kalbasi Aghbali, Amirala Baradaran, Behzad Bandehagh, Ali Delazar, Abbas Shahneh, Fatemeh Zare |
| AuthorAffiliation | 3 Immunology Research Center, Tabriz University of Medical Sciences, Daneshgah street 51666-14766, Tabriz, Iran 1 Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Daneshgah street 51666-14766, Tabriz, Iran 2 Drug Applied Research Center, Tabriz University of Medical Sciences, Daneshgah street 51666-14766, Tabriz, Iran |
| AuthorAffiliation_xml | – name: 1 Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Daneshgah street 51666-14766, Tabriz, Iran – name: 3 Immunology Research Center, Tabriz University of Medical Sciences, Daneshgah street 51666-14766, Tabriz, Iran – name: 2 Drug Applied Research Center, Tabriz University of Medical Sciences, Daneshgah street 51666-14766, Tabriz, Iran |
| Author_xml | – sequence: 1 givenname: Amirala surname: Aghbali fullname: Aghbali, Amirala organization: Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Daneshgah street 51666-14766, Tabriz, Iran – sequence: 2 givenname: Sepideh Vosough surname: Hosseini fullname: Hosseini, Sepideh Vosough – sequence: 3 givenname: Abbas surname: Delazar fullname: Delazar, Abbas – sequence: 4 givenname: Nader Kalbasi surname: Gharavi fullname: Gharavi, Nader Kalbasi – sequence: 5 givenname: Fatemeh Zare surname: Shahneh fullname: Shahneh, Fatemeh Zare – sequence: 6 givenname: Mona surname: Orangi fullname: Orangi, Mona – sequence: 7 givenname: Ali surname: Bandehagh fullname: Bandehagh, Ali – sequence: 8 givenname: Behzad surname: Baradaran fullname: Baradaran, Behzad |
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| SubjectTerms | Anticancer Antineoplastic Agents - pharmacology Apoptosis Carcinoma, Squamous Cell - pathology Care and treatment Cell Line, Tumor Cell Proliferation - drug effects cytotoxicity DNA Fragmentation Drug Screening Assays, Antitumor Grape seed extract Grape Seed Extract - pharmacology Health aspects Human Umbilical Vein Endothelial Cells Humans Mouth cancer Mouth Neoplasms - pathology oral squamous cell carcinoma Squamous cell carcinoma Vitis - chemistry |
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| Title | Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma |
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