Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma

• Published in: Biomolecules & biomedicine Vol. 13; no. 3; pp. 186 - 191
• Main Authors: Aghbali, Amirala, Hosseini, Sepideh Vosough, Delazar, Abbas, Gharavi, Nader Kalbasi, Shahneh, Fatemeh Zare, Orangi, Mona, Bandehagh, Ali, Baradaran, Behzad
• Format: Journal Article
• Language: English
• Published: Bosnia and Herzegovina Association of Basic Medical Sciences 01.08.2013; Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
• Subjects: Anticancer; Antineoplastic Agents - pharmacology; Apoptosis; Carcinoma, Squamous Cell - pathology; Care and treatment; Cell Line, Tumor; Cell Proliferation - drug effects; cytotoxicity; DNA Fragmentation; Drug Screening Assays, Antitumor; Grape seed extract; Grape Seed Extract - pharmacology; Health aspects; Human Umbilical Vein Endothelial Cells; Humans; Mouth cancer; Mouth Neoplasms - pathology; oral squamous cell carcinoma; Squamous cell carcinoma; Vitis - chemistry; Bosnia and Herzegovina
• ISSN: 1512-8601; 2831-0896; 1840-4812; 2831-090X
• DOI: 10.17305/bjbms.2013.2360

Development of novel therapeutic modalities is crucial for the treatment of oral squamous cell carcinoma (OSCC). Recent scientific studies have been focused on herbal medicines as potent anti-cancer drug candidates. This study is the first to investigate the cytotoxic effects and the mechanism of cell death induced by grape seed extract (GSE) in oral squamous cell carcinoma (KB cells). MTT (3-(4,5-dimetylthiazol-2-yl)-2,5 diphenyltetrazolium bromide) and trypan blue assays were performed in KB cells as well as human umbilical vein endothelial cells (HUVEC) were used to analyze the cytotoxic activity of GSE. Furthermore, the apoptosis-inducing action of the extract was determined by TUNEL, DNA fragmentation and cell death analysis. Statistical significance was determined by analysis of variance (ANOVA), followed by Duncan's test at a significance level of P≤0.05. The results showed apoptotic potential of GSE, confirmed by significant inhibition of cell growth and viability in a dose- and time- dependent manner without inducing damage to non-cancerous cell line HUVEC. The results of this study suggest that this plant contains potential bioactive compound(s) for the treatment of oral squamous cell carcinoma.