NIR fluorescence for monitoring in vivo scaffold degradation along with stem cell tracking in bone tissue engineering

Stem cell-based tissue engineering has the potential to use as an alternative for autologous tissue grafts; however, the contribution of the scaffold degradation along with the transplanted stem cells to in vivo tissue regeneration remains poorly understood. Near-infrared (NIR) fluorescence imaging...

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Bibliographic Details
Published inBiomaterials Vol. 258; p. 120267
Main Authors Kim, Soon Hee, Park, Ji Hoon, Kwon, Jin Seon, Cho, Jae Gu, Park, Kate G., Park, Chan Hum, Yoo, James J., Atala, Anthony, Choi, Hak Soo, Kim, Moon Suk, Lee, Sang Jin
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 01.11.2020
Subjects
Animals
Biodegradation
Bone Regeneration
Cell Tracking
Female
Hydrogels
Imaging
NIR fluorescence
Pregnancy
Rats
Scaffold
Stem Cells
Tissue Engineering
Tissue Scaffolds
Biodegradation
Scaffold
Tissue engineering
Imaging
NIR fluorescence
Stem cells
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Summary:Stem cell-based tissue engineering has the potential to use as an alternative for autologous tissue grafts; however, the contribution of the scaffold degradation along with the transplanted stem cells to in vivo tissue regeneration remains poorly understood. Near-infrared (NIR) fluorescence imaging has great potential to monitor implants while avoiding autofluorescence from the adjacent host tissue. To utilize NIR imaging for in vivo monitoring of scaffold degradation and cell tracking, we synthesized 800-nm emitting NIR-conjugated PCL-ran-PLLA-ran-PGA (ZW-PCLG) copolymers with three different degradation rates and labeled 700-nm emitting lipophilic pentamethine (CTNF127) on the human placental stem cells (CT-PSCs). The 3D bioprinted hybrid constructs containing the CT-PSC-laden hydrogel together with the ZW-PCLG scaffolds demonstrate that NIR fluorescent imaging enables tracking of in vivo scaffold degradation and stem cell fate for bone regeneration in a rat calvarial bone defect model. This NIR-based monitoring system can be effectively utilized to study cell-based tissue engineering applications.
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These authors contributed equally.
Soon Hee Kim: Methodology, Formal Analysis, Investigation, Writing – Original Draft, Visualization Ji Hoon Park: Methodology, Investigation, Writing – Original Draft Jin Seon Kwon: Methodology, Investigation Jae Gu Cho: Investigation Kate G. Park: Investigation Chan Hum Park: Formal Analysis James J. Yoo: Methodology, Resources, Funding Acquisition Anthony Atala: Resources, Funding Acquisition Hak Soo Choi: Conceptualization, Methodology, Resources, Writing – Review & Editing, Funding Acquisition Moon Suk Kim: Conceptualization, Methodology, Resources, Writing – Review & Editing, Supervision, Project Administration, Funding Acquisition Sang Jin Lee: Conceptualization, Methodology, Formal Analysis, Resources, Writing – Review & Editing, Visualization, Supervision, Project Administration, Funding Acquisition
CRediT Author Statement
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2020.120267