Bombyx mori Nucleopolyhedrovirus (BmNPV) Induces G2/M Arrest to Promote Viral Multiplication by Depleting BmCDK1

• Published in: Insects (Basel, Switzerland) Vol. 12; no. 12; p. 1098
• Main Authors: Xiao, Qin, Dong, Zhan-Qi, Zhu, Yan, Zhang, Qian, Yang, Xiu, Xiao, Miao, Chen, Peng, Lu, Cheng, Pan, Min-Hui
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08.12.2021; MDPI
• Subjects: Antibodies; Apoptosis; Baculoviridae; Baculovirus; BmCDK1; BmNPV; BmNPV iap1; Bombyx mori; Cell cycle; Cell growth; Cell proliferation; Cloning; Cyclin-dependent kinases; Disease resistance; DNA biosynthesis; Enzymes; Flow cytometry; Gene amplification; Gene expression; Immunoprecipitation; Infections; Insect cells; Insects; Kinases; Labelling; Multiplication; Pathogenesis; Proteins; Replication; Silkworms; Tubulin; Viruses; United States > US; China; Germany; Bombyx mori; BmNPV iap1; BmCDK1; BmNPV; Cell cycle
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects12121098

Understanding virus-host interaction is very important for delineating the mechanism involved in viral replication and host resistance. Baculovirus, an insect virus, can cause S or G2/M phase arrest in insect cells. However, the roles and mechanism of Baculovirus-mediated S or G2/M phase arrest are not fully understood. Our results, obtained using flow cytometry (FCM), tubulin-labeling, BrdU-labeling, and CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS), showed that Bombyx mori nucleopolyhedrovirus (BmNPV) induced G2/M phase arrest and inhibited cellular DNA replication as well as cell proliferation in BmN-SWU1 cells. We found that BmNPV induced G2/M arrest to support its replication and proliferation by reducing the expression of and . Co-immunoprecipitation assays confirmed that BmNPV IAP1 interacted with BmCDK1. BmNPV was involved in the process of BmNPV-induced G2/M arrest by reducing the content of BmCDK1. Taken together, our results improve the understanding of the virus-host interaction network, and provide a potential target gene that connects apoptosis and the cell cycle.