Effects of antimutagenic flavourings on SCEs induced by chemical mutagens in cultured Chinese hamster cells

• Published in: Mutation Research/Genetic Toxicology Vol. 189; no. 3; pp. 313 - 318
• Main Authors: Sasaki, YūF., Imanishi, Hisako, Ohta, Toshihiro, Shirasu, Yasuhiko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.1987
• Subjects: Animals; Anisaldehyde; Benzaldehydes - pharmacology; Cell Line; Cinnamaldehyde; Coumarin; Cricetinae; Cricetulus; Cultured Chinese hamster cells; Drug Interactions; Ethylvanillin; Female; Fibroblasts - drug effects; Flavoring Agents - pharmacology; Flavourings; Mitomycin C; Mutagens - pharmacology; Mutation; Ovary; Sister Chromatid Exchange - drug effects; Structure-Activity Relationship; Umbelliferone; Vanillin; Anisaldehyde; 4NQO; Cinnamaldehyde; ENNG; MMC; Umbelliferone; Cultured Chinese hamster cells; EMS; Flavourings; Coumarin; ENU; MMS; Ethylvanillin; SCE; MNU; Vanillin; Mitomycin C; BUdR; MNNG
• ISSN: 0165-1218; 0027-5107
• DOI: 10.1016/0165-1218(87)90063-2

Effects of antimutagenic flavourings such as vanillin, ethylvanillin, anisaldehyde, cinnamaldehyde, coumarin and umbelliferone on the induction of SCEs by MMC were investigated in cultured Chinese hamster ovary cells. None of these 6 flavourings showed any SCE-inducing activity by themselves. However, an obvious increase in the frequencies of SCEs was observed when MMC-pretreated cells were cultured in the presence of each flavouring. All these compounds have either an α, β-unsaturated carbonyl group or a carbonyl functionality neighbouring the phenyl group which may react with an enzyme SH-group and cause higher-order structure changes. SCE-enhancing effects of vanillin were further investigated on 6 other kinds of mutagens. Vanillin was also effective on SCEs induced by EMS, ENNG, ENU or MNU. On the other hand, MMS- or MNNG-induced SCEs were not influenced at all by vanillin. SCE-enhancing effects of vanillin seemed to be dependent on the quality of lesions in DNA.