A New Mitochondrial Genome of Sogatella furcifera (Horváth) (Hemiptera: Delphacidae) and Mitogenome-Wide Investigation on Polymorphisms

• Published in: Insects (Basel, Switzerland) Vol. 12; no. 12; p. 1066
• Main Authors: Park, Jongsun, Xi, Hong, Park, Jonghyun, Seo, Bo Yoon
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.11.2021; MDPI
• Subjects: Cox3 protein; Delphacidae; Genomes; Heteroplasmy; Insects; Insertion; intraspecific variations; Markers; Mitochondria; mitochondrial genome; Nucleotides; Pests; phylogenetic analysis; Phylogenetics; Phylogeny; Population genetics; Population studies; Populations; Simple sequence repeats; Single-nucleotide polymorphism; Sogatella; Sogatella furcifera; Species; New Zealand; South Korea; China; intraspecific variations; heteroplasmy; mitochondrial genome; phylogenetic analysis; Sogatella furcifera; simple sequence repeats
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects12121066

White-backed planthopper (WBPH), (Horváth), is one of the major sap-sucking rice pests in East Asia. We have determined a new complete mitochondrial genome of WBPH collected in the Korean peninsula using NGS technology. Its length and GC percentages are 16,613 bp and 23.8%, respectively. We observed one polymorphic site, a non-synonymous change, in the gene with confirmation heteroplasmy phenomenon within individuals of WBPH by PCR amplification and Sanger sequencing, the first report in this species. In addition, this heteroplasmy was not observed in wild WBPH populations, suggesting that it may be uncommon in fields. We analyzed single nucleotide polymorphisms, insertion, and deletions, and simple sequence repeats among the three WBPH mitogenomes from Korea and China and found diverse intraspecific variations, which could be potential candidates for developing markers to distinguish geographical populations. Phylogenetic analysis of 32 mitogenomes of Delphacidae including the three WBPH mitogenomes suggested that Delphacinae seems to be monophyletic and species including WBPH are clearly formed as one clade. In the future, it is expected that complete mitogenomes of individuals of geographically dispersed WBPH populations will be used for further population genetic studies to understand the migration pathway of WBPH.