Amperometric biosensors based on nafion coated screen-printed electrodes for the determination of cholinesterase inhibitors

Screen-printed electrodes coated with the nafion layer have been investigated for cholinesterase biosensor design. The butyrylcholinesterase (ChE) from horse serum was immobilised onto the nafion layer by cross-linking with glutaraldehyde vapours. The biosensors obtained showed better long-term stab...

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Bibliographic Details
Published inTalanta (Oxford) Vol. 53; no. 2; pp. 379 - 389
Main Authors Gogol, E.V., Evtugyn, G.A., Marty, J.-L., Budnikov, H.C., Winter, V.G.
Format Journal Article
LanguageEnglish
Russian
Published Amsterdam Elsevier B.V 01.11.2000
Oxford Elsevier
Subjects
Amperometric sensors
Biosensor
Cholinesterase
Crosslinking
Electrochemical electrodes
Pesticide determination
Pesticides
Screen-printed
Vapors
Pesticide determination
Screen-printed
Biosensor
Cholinesterase
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Summary:Screen-printed electrodes coated with the nafion layer have been investigated for cholinesterase biosensor design. The butyrylcholinesterase (ChE) from horse serum was immobilised onto the nafion layer by cross-linking with glutaraldehyde vapours. The biosensors obtained showed better long-term stability and lower working potential in comparison to those obtained with no nafion coating. The sensitivity of a biosensor toward organophosphate pesticides is not affected by the nafion coating. The detection limits were found to be 3.5×10 −7 M for trichlorfon and 1.5×10 −7 M for coumaphos.
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ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(00)00507-5