Suppression of progenitor differentiation requires the long noncoding RNA ANCR

Long noncoding RNAs (lncRNAs) regulate diverse processes, yet a potential role for lncRNAs in maintaining the undifferentiated state in somatic tissue progenitor cells remains uncharacterized. We used transcriptome sequencing and tiling arrays to compare lncRNA expression in epidermal progenitor pop...

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Bibliographic Details
Published inGenes & development Vol. 26; no. 4; pp. 338 - 343
Main Authors Kretz, Markus, Webster, Dan E, Flockhart, Ross J, Lee, Carolyn S, Zehnder, Ashley, Lopez-Pajares, Vanessa, Qu, Kun, Zheng, Grace X Y, Chow, Jennifer, Kim, Grace E, Rinn, John L, Chang, Howard Y, Siprashvili, Zurab, Khavari, Paul A
Format Journal Article
LanguageEnglish
Published United States Cold Spring Harbor Laboratory Press 15.02.2012
Subjects
Cell Differentiation
Cells, Cultured
Epidermal Cells
Gene Expression Regulation, Developmental
Keratinocytes - cytology
Research Communication
RNA Interference
RNA, Long Noncoding
RNA, Untranslated - genetics
RNA, Untranslated - metabolism
Stem Cells - cytology
Transcriptome
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Summary:Long noncoding RNAs (lncRNAs) regulate diverse processes, yet a potential role for lncRNAs in maintaining the undifferentiated state in somatic tissue progenitor cells remains uncharacterized. We used transcriptome sequencing and tiling arrays to compare lncRNA expression in epidermal progenitor populations versus differentiating cells. We identified ANCR (anti-differentiation ncRNA) as an 855-base-pair lncRNA down-regulated during differentiation. Depleting ANCR in progenitor-containing populations, without any other stimuli, led to rapid differentiation gene induction. In epidermis, ANCR loss abolished the normal exclusion of differentiation from the progenitor-containing compartment. The ANCR lncRNA is thus required to enforce the undifferentiated cell state within epidermis.
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These authors contributed equally to this work.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.182121.111