Carbon dioxide action on ethylene biosynthesis of preclimacteric and climacteric pear fruit

• Published in: Journal of experimental botany Vol. 54; no. 387; pp. 1537 - 1544
• Main Authors: de Wild, Hans P. J., Otma, Els C., Peppelenbos, Herman W.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.06.2003; OXFORD UNIVERSITY PRESS; Oxford Publishing Limited (England)
• Subjects: 1-aminocyclopropane-1-carboxylic acid oxidase; 1‐MCP; acc oxidase; Agronomy. Soil science and plant productions; Amino Acid Oxidoreductases - metabolism; Amino Acids, Cyclic - metabolism; apple fruit; Biological and medical sciences; Biosynthesis; Carbon dioxide; Carbon Dioxide - pharmacology; Cell Respiration - drug effects; Cell Respiration - physiology; Cyclopropanes - pharmacology; Desiccators; Economic plant physiology; Enzymes; Ethylene production; Ethylenes - biosynthesis; expression; feedback regulation; Fructification and ripening; Fructification, ripening. Postharvest physiology; Fruit - drug effects; Fruit - metabolism; Fundamental and applied biological sciences. Psychology; Gene expression regulation; Growth and development; inhibitors; Key words: Enzyme kinetics; metabolism; Models, Biological; Oxidases; Oxygen - metabolism; oxygen consumption; Plant physiology and development; plants; Postharvest technology; Pretreatment; Pyrus - drug effects; Pyrus - metabolism; Pyrus communis L; Research Papers: Cell and Molecular Biology, Biochemistry and Molecular Physiology; Respiration; suppression; synthase; Vegetative and sexual reproduction, floral biology, fructification; Pyrus communis L; Pyrus communis; Fruit; Rosaceae; Carbon dioxide; Ethylene; respiration; Ripening; Regulation(control); oxygen consumption; Dicotyledones; 1-MCP; Angiospermae; Plant growth substance; Spermatophyta; model; Fruit tree; Enzyme kinetics; feedback regulation
• ISSN: 0022-0957; 1460-2431; 1460-2431
• DOI: 10.1093/jxb/erg159

Ethylene production in pear fruit was studied at 2 °C. Several observations showed that the inhibiting effect of CO2 on ethylene production did not operate only via the binding site of the ethylene binding protein. Ethylene production of freshly harvested pears was stimulated by 1‐methylcyclopropene (1‐MCP), but unaffected or inhibited by CO2 which points to different action sites for both molecules. In climacteric pears, where ethylene production was strongly inhibited by 1‐MCP, a range of applied CO2 partial pressures was able to inhibit ethylene production further, to an extent similar to untreated pears. In the case of pears that had been stored for a period of 25 weeks, CO2 only had a clear effect after 1‐MCP pretreatment. Respiration measurements showed that the effect of CO2 on ethylene production did not operate via an effect on respiration. Ethylene production models based on measurements of whole pears were used to study CO2 effects. Kinetic parameters derived from the models point to the conversion from ACC to ethylene by ACC oxidase as a possible action site for CO2 inhibition.