Live imaging of neurogenesis in the adult mouse hippocampus

Neural stem and progenitor cells (NSPCs) generate neurons throughout life in the mammalian hippocampus. We used chronic in vivo imaging and followed genetically labeled individual NSPCs and their progeny in the mouse hippocampus for up to 2 months. We show that NSPCs targeted by the endogenous Achae...

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Published inScience (American Association for the Advancement of Science) Vol. 359; no. 6376; pp. 658 - 662
Main Authors Pilz, Gregor-Alexander, Bottes, Sara, Betizeau, Marion, Jörg, David J., Carta, Stefano, April, Simon, Simons, Benjamin D., Helmchen, Fritjof, Jessberger, Sebastian
Format Journal Article
LanguageEnglish
Published United States The American Association for the Advancement of Science 09.02.2018
Subjects
Animals
ASCL1 protein
Basic Helix-Loop-Helix Transcription Factors - genetics
Brain
Cell Death
Cell Division - genetics
Cells (biology)
Circuits
Dentate gyrus
Hippocampus
Hippocampus - cytology
Hippocampus - growth & development
Homology
Learning
Memory
Mice
Nerve Net - cytology
Nerve Net - growth & development
Neural stem cells
Neural Stem Cells - cytology
Neurogenesis
Neurogenesis - genetics
Neuroglia - cytology
Neuroimaging
Neurons
Neurons - cytology
Progenitor cells
Progeny
Promoter Regions, Genetic
Rodents
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Summary:Neural stem and progenitor cells (NSPCs) generate neurons throughout life in the mammalian hippocampus. We used chronic in vivo imaging and followed genetically labeled individual NSPCs and their progeny in the mouse hippocampus for up to 2 months. We show that NSPCs targeted by the endogenous Achaete-scute homolog 1 (Ascl1) promoter undergo limited rounds of symmetric and asymmetric divisions, eliciting a burst of neurogenic activity, after which they are lost. Further, our data reveal unexpected asymmetric divisions of nonradial glia-like NSPCs. Cell fates of Ascl1-labeled lineages suggest a developmental-like program involving a sequential transition from a proliferative to a neurogenic phase. By providing a comprehensive description of lineage relationships, from dividing NSPCs to newborn neurons integrating into the hippocampal circuitry, our data offer insight into how NSPCs support life-long hippocampal neurogenesis.
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These authors contributed equally.
ISSN:0036-8075
1095-9203
1095-9203
DOI:10.1126/science.aao5056