A More Oxidized Plasma Albumin Redox State and Lower Plasma HDL Particle Number Reflect Low-Protein Diet Ingestion in Adult Rats

• Published in: The Journal of nutrition Vol. 150; no. 2; pp. 256 - 266
• Main Authors: Wada, Yasuaki, Izumi, Hirohisa, Shimizu, Takashi, Takeda, Yasuhiro
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2020; Oxford University Press; American Institute of Nutrition
• Subjects: Albumin; Albumins; Animals; biomarker; Biomarkers; Biomarkers - blood; Blood Proteins - metabolism; Body mass; Casein; Diet; Diet, Protein-Restricted; Fibroblast Growth Factors - metabolism; Gastrocnemius muscle; HDL; High density lipoprotein; High-performance liquid chromatography; Ingestion; Insulin-Like Growth Factor I - metabolism; Lipids; Lipoproteins, HDL - metabolism; Liquid chromatography; Low protein diet; Male; mercaptalbumin; Muscles; Nutrient deficiency; Nutrition; Nutritional status; Oxidation-Reduction; Plasma; plasma albumin redox state; protein undernutrition; Proteins; proteomics; Rats; Rats, Wistar; Redox properties; Reproducibility of Results; Serum Albumin - metabolism; skeletal muscle mass; Transthyretin; CRP; protein undernutrition; LP; mercaptalbumin; PON1; ACTB; KNG1; HDL; TTR; MA; EIF4EBP1; HMOX1; BUN; Cys; GSH; FGF21; GSSG; RBP; proteomics; transthyretin; plasma albumin redox state; APOA2; CK; TBARS; CN; IGF1; biomarker; skeletal muscle mass; AOPP; CT; TF; NA; albumin; ALB; PEM; p-EIF4EBP1
• ISSN: 0022-3166; 1541-6100
• DOI: 10.1093/jn/nxz223

Plasma albumin (ALB) redox state reflects protein nutritional status, but how it differs from other protein nutrition biomarkers remains to be fully elucidated. This study aimed to delineate the characteristics of plasma ALB redox state as a protein nutrition biomarker. Adult male Wistar rats were maintained on an AIN-93 M [14% casein, control (CT)] diet or an AIN-93 M-based 5% casein [low protein (LP)] diet ad libitum for 4 wk. Plasma samples were repeatedly obtained from the same rats at weeks 0–4, ALB redox state was determined by HPLC, and the concentrations of conventional protein nutrition biomarkers, ALB and transthyretin (TTR), were compared between the groups by Student t test. Body mass, relative muscle masses, plasma proteome, and plasma lipids at week 4 were also compared. Plasma ALB redox state shifted to a more oxidized state in the LP diet group compared with the CT diet group at weeks 1–4. The LP diet group also showed significantly lower plasma ALB concentrations at weeks 1 and 2 (13% and 11% lower, respectively) and significantly lower TTR concentration at week 1 (21% lower) compared with the CT diet group, but these concentrations did not differ significantly at weeks 3 and 4. After 4 wk, body mass and relative soleus and gastrocnemius muscle masses did not differ, but the relative plantaris muscle mass tended to be 4% lower (1.75 compared with 1.68 g/kg body mass) in the LP diet group compared with the CT group (P = 0.06). The LP diet group also had a significantly lower HDL particle number than the CT group (30% lower). A more oxidized plasma ALB redox state and lower plasma HDL particle number reflect LP diet ingestion in adult rats, which did not exhibit changes of plasma ALB and TTR concentrations.