Isolation and algae-lysing characteristics of the algicidal bacterium B5

• Published in: Journal of environmental sciences (China) Vol. 19; no. 11; pp. 1336 - 1340
• Main Authors: MU, Rui-min, FAN, Zheng-qiu, PEI, Hai-yan, YUAN, Xue-liang, Liu, Si-xiu, WANG, Xiang-rong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 2007
• Subjects: 16S rDNA; algae-lysing characteristic; algicidal bacteria; Bacillus; Bacillus fusiformis; Carbon; Cell Count; Cell Death; Chlorella; Chlorella - cytology; Chlorella - growth & development; Chlorophyll - metabolism; Chlorophyll A; Databases, Nucleic Acid; DNA, Ribosomal - genetics; Eutrophication; Microcystis - cytology; Microcystis - growth & development; Microcystis aeruginosa; RNA, Ribosomal, 16S - genetics; Scenedesmus; Scenedesmus - cytology; Scenedesmus - growth & development; Sequence Analysis, DNA; Sewage - microbiology; water blooms; 溶藻细菌; 细胞溶解; 藻类水华; algicidal bacteria; water blooms; 16S rDNA; algae-lysing characteristic; Bacillus fusiformis
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/S1001-0742(07)60218-6

Water blooms have become a worldwide environmental problem. Recently, algicidal bacteria have attracted wide attention as possible agents for inhibiting algal water blooms. In this study, one strain of algicidal bacterium B5 was isolated from activated sludge. On the basis of analysis of its physiological characteristics and 16S rDNA gene sequence, it was identified as Bacillusfusiformis. Its algaelysing characteristics on Microcystis aeruginosa, Chlorella and Scenedesmus were tested. The results showed that： （1） the algicidal bacterium B5 is a Gram-negative bacterium. The 16S rDNA nucleotide sequence homology of strain B5 with 2 strains of B. fusiformis reached 99.86%, so B5 was identified as B. fusiformis; （2） the algal-lysing effects of the algicidal bacterium B5 on M. aeruginosa, Chlorella and Scenedesrnus were pronounced. The initial bacterial and algal cell densities strongly influence the removal rates of chlorophyll-a. The greater the initial bacterial cell density, the faster the degradation of chlorophyll-a. The greater the initial algal cell density, the slower the degradation of chlorophyll-a. When the bacterial cell density was 3.6 × 10^7 cells/ml, nearly 90% of chlorophyll-a was removed. When the chlorophyll-a concentration was less than 550 μg/L, about 70% was removed; （3） the strain B5 lysed algae by secreting metabolites and these metabolites could bear heat treatment.