Immunodetection of NGF, trkA, p75 and inhibin alpha-subunit in interstitial cells of golden hamsters treated with hCG

• Published in: Journal of Reproduction and Development Vol. 55; no. 6; pp. 622 - 628
• Main Authors: Weng, Q., Beijing Forestry Univ. (China), Shi, Z.Q, Tukada, J, Watanabe, G, Taya, K
• Format: Journal Article
• Language: English
• Published: Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 01.12.2009
• Subjects: Animals; CELLS; CELLULE; CELULAS; Chorionic Gonadotropin - pharmacology; Cricetinae; FACTEUR DE CROISSANCE; FACTORES DE CRECIMIENTO; Female; Golden hamster; Gonadal Steroid Hormones - blood; GONADOTROPHINE; GONADOTROPINAS; GONADOTROPINS; Gonadotropins - blood; GROWTH FACTORS; HAMSTER; HAMSTERS; http://www.fao.org/aos/agrovoc#c_1418; http://www.fao.org/aos/agrovoc#c_3329; http://www.fao.org/aos/agrovoc#c_3482; http://www.fao.org/aos/agrovoc#c_36557; http://www.fao.org/aos/agrovoc#c_4304; http://www.fao.org/aos/agrovoc#c_49900; http://www.fao.org/aos/agrovoc#c_5137; http://www.fao.org/aos/agrovoc#c_5456; Immunohistochemistry; Inhibin α-subunit; Inhibins - blood; Inhibins - metabolism; Interstitial cell; Mesocricetus; NERF; Nerve growth factor (NGF); Nerve Growth Factor - metabolism; NERVES; NERVIOS; OVAIRE; OVARIES; OVARIOS; Ovary - cytology; Ovary - drug effects; Ovary - metabolism; Ovulation Induction; p75 and trkA; RECEPTEURS; RECEPTOR; Receptor, Nerve Growth Factor - metabolism; Receptor, trkA - metabolism; Theca Cells - cytology; Theca Cells - drug effects; Theca Cells - metabolism; Time Factors
• ISSN: 0916-8818; 1348-4400
• DOI: 10.1262/jrd.20208

The aim of this study was to investigate the effect of luteinizing hormone (LH) surge on expression of nerve growth factor (NGF), trkA, p75 and inhibin alpha-subunit in ovarian interstitial cells of golden hamsters after human chorionic gonadotropin (hCG) treatment. NGF, two NGF receptors (trkA, p75) and inhibin alpha-subunit were immunolocalized by immunohistochemistry, and gonadotropins, steroid hormones and immunoreactive (ir-) inhibin concentrations were measured by radioimmunoassay. Stronger positive staining of NGF, trkA and p75 were found in interstitial cells at 6, 12 and 18 h after hCG injection in the treated group compared with the control groups. Inhibin alpha-subunit staining was found in interstitial cells at 12 and 18 h after hCG injection in the treatment group, but not in the control group. Plasma concentrations of progesterone increased significantly from 6 to 18 h after hCG treatment, whereas plasma concentrations of estradiol-17beta significantly decreased compared with the control group. An increased plasma concentration of FSH and decreased concentrations of ir-inhibin from 6 to 18 h after hCG treatment verified the negative relationship. There were no significant changes in the concentrations of LH in the hCG-treated group. In contrast, the typical preovulatory LH surge was found at 1700 h on day 4 (proestrus) in the control group. These results suggested that LH surge can induce expression of NGF, trkA, p75 and inhibin alpha-subunit in ovarian interstitial cells and that NGF, trkA, p75 and inhibin alpha-subunit may have a paracrine or autocrine role in modulation of ovarian interstitial cell function in golden hamsters.