A plant lipocalin promotes retinal-mediated oscillatory lateral root initiation

In , de novo organogenesis of lateral roots is patterned by an oscillatory mechanism called the root clock, which is dependent on unidentified metabolites. To determine whether retinoids regulate the root clock, we used a chemical reporter for retinaldehyde (retinal)–binding proteins. We found that...

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Published inScience (American Association for the Advancement of Science) Vol. 373; no. 6562; pp. 1532 - 1536
Main Authors Dickinson, Alexandra J, Zhang, Jingyuan, Luciano, Michael, Wachsman, Guy, Sandoval, Evan, Schnermann, Martin, Dinneny, José R, Benfey, Philip N
Format Journal Article
LanguageEnglish
Published United States The American Association for the Advancement of Science 24.09.2021
Subjects
Arabidopsis
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
Arabidopsis Proteins - chemistry
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Fluorescence
Homology
Lipocalin
Lipocalins - chemistry
Lipocalins - genetics
Lipocalins - metabolism
Meristem - metabolism
Metabolites
Mutation
Nutrients
Organogenesis
Organogenesis, Plant
Oscillations
Plant Roots - growth & development
Plant Roots - metabolism
Protein Binding
Proteins
Pyrimidinones - metabolism
Retina
Retinal-binding protein
Retinaldehyde
Retinaldehyde - metabolism
Retinaldehyde - pharmacology
Retinoids
Roots
Signal Transduction
Vertebrates
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Summary:In , de novo organogenesis of lateral roots is patterned by an oscillatory mechanism called the root clock, which is dependent on unidentified metabolites. To determine whether retinoids regulate the root clock, we used a chemical reporter for retinaldehyde (retinal)–binding proteins. We found that retinal binding precedes the root clock and predicts sites of lateral root organogenesis. Application of retinal increased root clock oscillations and promoted lateral root formation. Expression of an protein with homology to vertebrate retinoid-binding proteins, TEMPERATURE INDUCED LIPOCALIN (TIL), oscillates in the region of retinal binding to the reporter, confers retinal-binding activity in a heterologous system, and, when mutated, decreases retinal sensitivity. These results demonstrate a role for retinal and its binding partner in lateral root organogenesis.
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Author contributions: AJD and PNB conceived of the work and drafted the manuscript. AJD, PNB, JZ, ML, MS, GW, ESB, and JRD designed the experiments and interpreted the data. AJD, JZ, ML, GW, and ESB acquired the data. All authors revised the manuscript.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.abf7461