Catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus Sphingomonas for vigorous growth

• Published in: International journal of systematic and evolutionary microbiology Vol. 54; no. 3; pp. 955 - 959
• Main Authors: Tanaka, Yasuhiro, Hanada, Satoshi, Manome, Akira, Tsuchida, Takayasu, Kurane, Ryuichiro, Nakamura, Kazunori, Kamagata, Yoichi
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.05.2004; Society for General Microbiology
• Subjects: Bacterial plant pathogens; Bacteriology; Base Composition; Biological and medical sciences; Catellibacterium nectariphilum; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Microbiology; Microscopy, Electron; Miscellaneous; Molecular Sequence Data; Phenotype; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; Rhodobacteraceae - classification; Rhodobacteraceae - genetics; Rhodobacteraceae - growth & development; Rhodobacteraceae - metabolism; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sewage - microbiology; Sphingomonas - metabolism; Systematics; Bacteria; Sphingomonas; Strain
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.02750-0

1 Research Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan 2 Central Research Laboratories of Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-8186, Japan Correspondence Satoshi Hanada s-hanada{at}aist.go.jp A bacterial strain, designated AST4 T , was isolated from activated sludge. The bacterium did not show significant growth on nutrient broth, but growth was clearly stimulated by addition of supernatant from other bacterial cultures. Culture filtrate of a strain related to the genus Sphingomonas in particular increased the cell yield and growth rate of strain AST4 T . Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain AST4 T is located within the ‘ Rhodobacter group’ in the -3 subclass of Proteobacteria , but is clearly distant from related genera in this group such as Paracoccus , Rhodobacter and Rhodovulum . Strain AST4 T is a Gram-negative, non-motile, rod-shaped (0·6–0·8 x 1·3–2·0 µm) and aerobic bacterium. It was not able to reduce nitrate to nitrite or N 2 . No phototrophic growth was observed. Optimal growth occurred at 30 °C and pH 6·5–7·5. The dominant cellular fatty acid in the isolate was C 18 : 1 cis 11. Ubiquinone-10 was the major respiratory quinone. The G+C content was 64·5 mol% (by HPLC). Based on the phylogenetic and phenotypic traits, the name Catellibacterium nectariphilum gen. nov., sp. nov. is proposed for this isolate; the type strain is AST4 T (=NBRC 100046 T =JCM 11959 T =DSM 15620 T ). Published online ahead of print on 23 January 2004 as DOI 10.1099/ijs.0.02750-0. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains AST4 T and GF9 are AB101543 and AB101552 , respectively. An extended 16S rDNA-based neighbour-joining tree containing all species in the genera Paracoccus , Rhodobacter and Rhodovulum is available as supplementary material in IJSEM Online. Present address: Kankyo Engineering Corporation, 1 Kimitsu, Kimitsu-shi, Chiba 299-1141, Japan. Present address: Biotechnology Research Centers, KUBOTA Co., Inc., 5–6 Koyodai, Ryugasaki, Ibaraki 301-0852, Japan.