Changes in glycosidase activities during galactoglucomannan oligosaccharide inhibition of auxin induced growth

• Published in: Phytochemistry (Oxford) Vol. 65; no. 13; pp. 1903 - 1909
• Main Authors: Bilisics, Ladislav, Vojtaššák, Jozef, Capek, Peter, Kollárová, Karin, Lišková, Desana
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01.07.2004; Elsevier
• Subjects: 2,4-D; alpha-galactosidase; alpha-L-arabinosidase; auxins; beta-galactosidase; beta-glucosidase; beta-mannosidase; Biological and medical sciences; cell fractionation; Cell Wall - drug effects; Cell Wall - enzymology; cell walls; Chemical agents; Elongation growth; enzyme activity; Fabaceae; Fundamental and applied biological sciences. Psychology; galactoglucomannan; Galactoglucomannan oligosaccharides; Glycosidases; Glycoside Hydrolases - metabolism; Indoleacetic Acids - pharmacology; mannans; Mannans - chemistry; oligosaccharides; Oligosaccharides - pharmacology; peas; Pisum sativum; Pisum sativum - drug effects; Pisum sativum - growth & development; Pisum sativum L. cv. Tyrkys; plant extracts; plant growth control; Plant physiology and development; plant proteins; Plant Stems - drug effects; Plant Stems - growth & development; Vegetative apparatus, growth and morphogenesis. Senescence; xylan 1,4-beta-xylosidase; Galactoglucomannan oligosaccharides; Pisum sativum L. cv. Tyrkys; Glycosidases; Fabaceae; Elongation growth; Auxin; Enzyme; Growth; Oligosaccharide; Stem; Leguminosae; Enzymatic activity; Dicotyledones; Pisum sativum; Angiospermae; Hydrolases; Plant growth substance; Spermatophyta; Inhibition; Elongation
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2004.06.002

The inhibition of 2,4-D-induced elongation growth by galactoglucomannan oligosaccharides in pea stem segments ( Pisum sativum L. cv. Tyrkys) after 18 h of incubation results in changes of extracellular, intracellular and cell wall glycosidase activities (β- d-glucosidase, β- d-mannosidase, β- d-galactosidase, β- d-xylosidase, α- d-galactosidase, and α- l-arabinosidase). The inhibition of 2,4-D-induced elongation growth by galactoglucomannan oligosaccharides (GGMOs) in pea stem segments ( Pisum sativum L. cv. Tyrkys) after 18 h of incubation results in changes of extracellular, intracellular and cell wall glycosidase activities (β- d-glucosidase, β- d-mannosidase, β- d-galactosidase, β- d-xylosidase, α- d-galactosidase, and α- l-arabinosidase). GGMOs lowered the glycosidase activities in the extracellular fraction, while in the cell wall fractions their activities were markedly increased. The intracellular enzyme α- d-galactosidase increased while the β- d-galactosidase decreased in activity in response to the GGMO treatment. Extracellular enzymes showed low values of activities in comparison with intracellular and cell wall glycosidases. It is evident that GGMOs can alter auxin induced elongation and glycosidase activities in different compartments of the cell, however, the mode and site of their action remains unclear.