3-Hydroxy fatty acids in saliva as diagnostic markers in chronic periodontitis

• Published in: Journal of microbiological methods Vol. 62; no. 3; pp. 285 - 291
• Main Authors: Ferrando, Raúl, Szponar, Bogumila, Sánchez, Arturo, Larsson, Lennart, Valero-Guillén, Pedro L.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Shannon Elsevier B.V 01.09.2005; Elsevier Science
• Subjects: 3-hydroxy fatty; 3-Hydroxy fatty acids; acids; Adult; Anaerobic Gram-negative bacteria/periodontal pathogens; Bacterial diseases; Bacteriology; Basic Medicine; Biological and medical sciences; Biomarkers - analysis; Case-Control Studies; Chronic Disease; Ent and stomatologic bacterial diseases; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Fatty Acids - analysis; Fatty Acids - chemistry; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; gas chronnatography-mass spectrometry; Human bacterial diseases; Humans; Hydroxylation; inarkers; Infectious diseases; Medical and Health Sciences; Medical sciences; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; microbial chemical; Microbial chemical markers; Microbiology; Microbiology in the medical area; Middle Aged; Mikrobiologi inom det medicinska området; Molecular Structure; Non tumoral diseases; Otorhinolaryngology. Stomatology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Periodontitis - diagnosis; Periodontitis - metabolism; Periodontitis/etiology; Predictive Value of Tests; Saliva - chemistry; Sensitivity and Specificity; Anaerobic Gram-negative bacteria/periodontal pathogens; Microbial chemical markers; 3-Hydroxy fatty acids; Periodontitis/etiology; Gas chromatography–mass spectrometry; Stomatology; Lipids; Marker; Gas chromatography-mass spectrometry; Fatty acids; Gas chromatography; Periodontal disease; Chronic; Periodontitis; Etiology; Saliva; Mass spectrometry; Gram negative bacteria
• ISSN: 0167-7012; 1872-8359; 1872-8359
• DOI: 10.1016/j.mimet.2005.04.014

Saturated straight- and branched-chain 3-hydroxy fatty acids (3-OH FAs) of 10–18 carbon chain lengths were determined in saliva from 27 individuals with chronic periodontitis and 18 healthy individuals by using gas chromatography–tandem mass spectrometry. Of the 14 different 3-OH FAs detected, 3-OH-C i17:0 was the most abundant in the periodontitis samples while 3-OH-C 14:0 was the most abundant in the healthy individuals. Considering the relative percentages of 3-OH-C 12:0, 3-OH-C 14:0, 3-OH-C i17:0, and 3-OH-C 17:0, 95.6% of all cases were correctly classified as healthy individuals or periodontitis patients by means of discriminant analysis. The sensitivity, specificity, positive predictive value and negative predictive value of 3-OH FA analysis in diagnosing peridontitis were, respectively, 0.92, 1.00, 1.00, and 0.90. The results indicate that 3-OH FA analysis of saliva samples is a useful diagnostic method in chronic periodontitis.