Uric acid recycling in the shield bug, Parastrachia japonensis (Hemiptera: Parastrachiidae), during diapause

• Published in: Journal of insect physiology Vol. 52; no. 8; pp. 816 - 825
• Main Authors: Kashima, Takayuki, Nakamura, Tomoyasu, Tojo, Sumio
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2006
• Subjects: allantoic acid; allantoicase; allantoin; allantoinase; Animals; bacteria; Cecum - enzymology; Cecum - microbiology; Diapause; endosymbionts; enzyme activity; enzymes; Erwinia; Feces - microbiology; Female; Hemiptera; Heteroptera - metabolism; Heteroptera - microbiology; intestinal microorganisms; Male; Nitrogen - metabolism; Nymph - metabolism; Parastrachia japonensis; Parastrachiidae; reproductive diapause; Symbiont; Symbiosis - physiology; Urate Oxidase - metabolism; urea; uric acid; Uric Acid - metabolism; Uric acid recycling; Uricase; Uricolytic enzyme; uricolytic enzymes; Uricolytic enzyme; Uric acid recycling; Uricase; Symbiont; Diapause
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2006.05.003

Nymphs of the univoltine shield bug, Parastrachia japonensis grow by feeding on the drupes of their sole food plant, which are available for only 2 weeks a year. The new adults soon enter a reproductive diapause and survive without feeding for at least 10 months up to 2 years. Uric acid was found to be the predominant component among four waste nitrogenous compounds, i.e., uric acid, allantoin, allantoic acid and urea in the body of both nymphs and adults in all stages, and to be predominantly excreted by the nymphs and reproductive adults. However, adults in diapause excreted negligible amounts of these compounds. Erwinia-like bacteria were found exclusively in the cecum of midgut, in which three uricolytic enzymes, i.e., uricase, allantoinase and allantoicase were detected. Ninety % of adults in diapause could survive on water for 9 months, but those given 0.02% rifampicin aqueous solution all died within this period, with significant reduction of the bacteria and uricase activity in the cecum. Rifampicin treatment resulted in a considerable reduction of free amino acids, especially proline in the hemolymph. These results suggest that uric acid is recycled as an amino acid source with the aid of Erwinia-like bacteria, and uricase functions as a key enzyme for this process.