Random amplified polymorphic DNA markers for DNA fingerprinting and genetic variability assessment of minute parasitic wasp species (Hymenoptera: Mymaridae and Trichogrammatidae) used in biological control programs of phytophagous insects

Biological control of insects that feed on our crops has become more practical in recent years by mass release of egg parasitoid microhymenoptera. Trichogramma species are now commercially reared and spread in commercial fields to control specific insect pests. Microhymenoptera species are, however,...

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Bibliographic Details
Published inGenome Vol. 36; no. 3; p. 580
Main Authors Landry, B.S, Dextraze, L, Boivin, G
Format Journal Article
LanguageEnglish
Published Canada 01.06.1993
Subjects
ADN
ANAPHES
Animals
AUXILIAIRE DE LUTTE BIOLOGIQUE
Base Sequence
BIOTIPOS
BIOTYPE
DNA - genetics
DNA Fingerprinting
Genetic Markers
Genetic Variation
Male
MARCADORES GENETICOS
MARQUEUR GENETIQUE
Molecular Sequence Data
ORGANISMOS PARA CONTROL BIOLOGICO
PARASITOIDE
PARASITOIDES
Pest Control, Biological
Phylogeny
Polymerase Chain Reaction
Polymorphism, Genetic
Species Specificity
TRICHOGRAMMA DENDROLIMI
TRICHOGRAMMA EVANESCENS
TRICHOGRAMMA PRETIOSUM
VARIACION GENETICA
VARIATION GENETIQUE
Wasps - genetics
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Summary:Biological control of insects that feed on our crops has become more practical in recent years by mass release of egg parasitoid microhymenoptera. Trichogramma species are now commercially reared and spread in commercial fields to control specific insect pests. Microhymenoptera species are, however, very small and morphologically indistinguishable within species, although strains of a given species differ in their efficiency to control specific insect pests. Traditional taxonomy is unable to differentiate microhymenoptera species at the strain level. It is becoming increasingly important to develop a reliable system to monitor genetic variations both within and between strains of commercially important microhymenoptera, to detect genetic drift occurring during several generations of multiplication, to protect patents, and to certify the lots of commercially released microhymenoptera. We have developed a system based on DNA markers to rapidly characterize individuals of five species of microhymenoptera from the genus Anaphes and Trichogramma including a new species of Anaphes not previously described. The main components of our system are a rapid and simple DNA microextraction method and fast DNA polymorphism analyses based on random amplified polymorphic DNA markers
Bibliography:9500029
H10
ISSN:0831-2796
1480-3321
DOI:10.1139/g93-078