Glutamatergic and dopaminergic afferents to the prefrontal cortex regulate spatial working memory in rats

• Published in: Neuroscience Vol. 92; no. 1; pp. 97 - 106
• Main Authors: Romanides, A.J, Duffy, P, Kalivas, P.W
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.01.1999; Elsevier
• Subjects: Animals; Behavioral psychophysiology; Biological and medical sciences; dopamine; Dopamine - metabolism; Fundamental and applied biological sciences. Psychology; glutamate; Glutamic Acid - metabolism; Male; Maze Learning - physiology; mediodorsal thalamus; Memory - physiology; Neurons, Afferent - physiology; Neurotransmission and behavior; prefrontal cortex; Prefrontal Cortex - physiology; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Rats; Rats, Sprague-Dawley; Receptors, GABA-B - physiology; Receptors, Opioid, mu - physiology; Space life sciences; Space Perception - physiology; Synaptic Transmission - physiology; Thalamus - physiology; ventral tegmental area; working memory; VTA, ventral tegmental area; prefrontal cortex; CPP, 3-( R)-2-carboxypiperazin-4-propyl-1-phosphonic acid; DAMGO, [ d-Ala 2, N-Me-Phe 4,Gly-ol 5]enkephalin; MCPG, ( RS)-α-methyl-4-carboxyphenylglycine; DMSO, dimethylsulfoxide; ventral tegmental area; working memory; AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate; mediodorsal thalamus; CNQX, 6-cyano-7-nitroquinoxaline-2,3-dione; PFC, prefrontal cortex; dopamine; NMDA, N-methyl- d-aspartate; glutamate; Dopamine; Rat; Rodentia; Central nervous system; Prefrontal cortex; Catecholamine; Glutamate; Vertebrata; Mammalia; Animal; Excitatory aminoacid; Neurotransmitter; Thalamus; Working memory; Ventral tegmental area; Brain (vertebrata); Spatial memory
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/S0306-4522(98)00747-7

The integrity of the prefrontal cortex is critical for the expression of working memory. The prefrontal cortex is innervated by dopaminergic afferents from the ventral tegmental area and glutamatergic afferents from the mediodorsal thalamus. To determine the role of dopaminergic and glutamatergic afferents in the regulation of working memory, rats were trained to perform a spatial delayed alternation task in a T-maze. The microinjection of the ionotropic glutamate antagonists 6-cyano-7-nitroquinoxaline-2,3-dione or 3-( R)-2-carboxypiperazin-4-propyl-1-phosphonic acid into the prefrontal cortex impaired working memory. Consistent with a role for glutamate receptor activation, microinjecting the GABA B agonist baclofen into the mediodorsal thalamus produced a dose-dependent disruption of working memory. In contrast, inhibition of the mesocortical dopamine projection was without effect on working memory. The blockade of D 1 and/or D 2 dopamine receptors with SCH-23390 and sulpiride was without effect on working memory. Likewise, the microinjection of baclofen into the ventral tegmental area did not impair working memory. However, stimulating mu-opioid receptors in the ventral tegmental area with [ d-Ala 2, N-Me-Phe 4,Gly-ol 5]enkephalin produced a dose-dependent impairment of working memory that was reversed by blocking D 1 dopamine receptors with SCH-23390 in the prefrontal cortex. These data demonstrate that increased dopamine tone or reduced glutamate tone in the prefrontal cortex disrupts working memory in a spatial delayed alternation task.