Study on gelatin-containing artificial skin IV: a comparative study on the effect of antibiotic and EGF on cell proliferation during epidermal healing

• Published in: Biomaterials Vol. 22; no. 20; pp. 2777 - 2783
• Main Authors: Hong, Sung Ran, Lee, Seung Jun, Shim, Jong Won, Choi, Young Seon, Lee, Young Moo, Song, Kang Won, Park, Moon Hyang, Nam, Young Soo, Lee, Sang In
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2001; Elsevier Science
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Artificial Organs; Biological and medical sciences; Cell Division - drug effects; EGF; Epidermal Cells; Epidermal Growth Factor - pharmacology; Epidermis - drug effects; Epidermis - metabolism; Gelatin; Gelatin - analysis; Hyaluronate; Immunohistochemistry; Medical sciences; PC10; PCNA; Proliferating Cell Nuclear Antigen - metabolism; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Rats; Rats, Wistar; Skin - chemistry; Sponge; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Technology. Biomaterials. Equipments. Material. Instrumentation; Wound dressing; Wound Healing - drug effects; PCNA; Gelatin; EGF; PC10; Sponge; Wound dressing; Hyaluronate; Performance evaluation; Cell proliferation; Animal model; Artificial skin; Rat; Cytokine; Rodentia; Experimental study; Vertebrata; Antibiotic; Mammalia; Epidermal growth factor; Polypeptide; Healing agent; Animal; Biomaterial; Chemical composition; Hyaluronic acid; Comparative study; Growth factor
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(01)00021-7

Gelatin-hyaluronate sponge with and without antibiotic and epidermal growth factor (EGF) were prepared and compared. Four types of sponges were applied on the full-thickness dorsal skin defect of Wistar rat. The effects of antibiotic and EGF in gelatin-hyaluronate sponge on wound healing were investigated by light microscopy and image analyzer at postoperative days of 5, 12 and 21. An immunohistochemical technique, employing PC10, a monoclonal antibody against proliferating cell nuclear antigen (PCNA) was applied to wounded tissue sections. The number of PC10-positive cells was very high for the sponge with EGF at postoperative day 5, then gradually decreased with time. Also we found that antibiotics restrained the cell proliferation during the migratory phase. The sponge with both antibiotic and EGF showed good wound healing performances on the whole for a healing period. The epithelium was regenerated fast with EGF-impregnated sponges at day 5, but each sample had nearly the same length of regenerated epithelium at day 12.