Diversification of IFNγ-inducible CXCb chemokines in cyprinid fish

• Published in: Developmental and comparative immunology Vol. 38; no. 2; pp. 243 - 253
• Main Authors: van der Aa, Lieke M., Chadzinska, Magdalena, Derks, Wouter, Scheer, Marleen, Levraud, Jean-Pierre, Boudinot, Pierre, Lidy Verburg-van Kemenade, B.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.10.2012; Elsevier
• Subjects: Animals; carp; Carps - immunology; Carps - metabolism; chemokine CXCL9; Chemokines, CXC - genetics; chromosomes; Cloning, Molecular; CXCb chemokines; Cyprinids; Cyprinus carpio; Danio rerio; Evolution, Molecular; gene expression; genes; granulocytes; Interferon-gamma - immunology; Interferon-gamma - metabolism; interleukin-8; kidneys; Leukocyte stimulation; Leukocytes - immunology; Leukocytes - metabolism; Life Sciences; Lipopolysaccharides - metabolism; lymphocytes; macrophages; mammals; monocytes; Peritoneum - cytology; Peritoneum - immunology; peritonitis; Peritonitis - immunology; Promoter analysis; Promoter Regions, Genetic; tissues; Transcriptome; Zebrafish - genetics; Zebrafish - immunology; Zymosan; Leukocyte stimulation; CXCb chemokines; Promoter analysis; Cyprinids; CELLS; DIFFERENTIAL EXPRESSION; MOLECULAR EVOLUTION; CHEMOATTRACTANT I-TAC; EARLY-RESPONSE; CUTTING EDGE; CARPIO L; GENE-EXPRESSION; TROUT ONCORHYNCHUS-MYKISS; RECEPTOR CXCR3
• ISSN: 0145-305X; 1879-0089; 1879-0089
• DOI: 10.1016/j.dci.2012.05.005

We earlier identified two CXCL8-like lineages in cyprinid fish, which are functional homologues of the mammalian CXCL8, but with diverged functions. We here investigated whether the carp IFN-γ-inducible CXCb gene, related to the mammalian CXCL9, -10 and -11 chemokines, was subject to a similar diversification. On the zebrafish genome, a cluster of seven CXCb genes was found on chromosome five. Analysis of the promoter of the zebrafish CXCb genes suggests a partially shared, but differential induction. A second CXCb gene, CXCb2, was identified in common carp by homology cloning. CXCb2 is constitutively expressed in immune-related tissues, predominantly in head kidney lymphocytes/monocytes. Interestingly, an induction of CXCb2 gene expression with recombinant carp IFN-γ2 and LPS was observed in macrophages and granulocytes. Finally, difference in sensitivity to LPS, and kinetics of CXCb1 and CXCb2 gene expression during zymosan-induced peritonitis, was observed. These results indicate a functional diversification for cyprinid CXCb chemokines, with functional homology to mammalian CXCL9–11.