A novel real time PCR assay using melt curve analysis for ivory identification

Highlights • The real-time PCR based assay was proposed for ivory species identification. • Three extant elephant species were clearly classified by the developed assay. • The assay was fully validated according to ISFG recommendation. • The assay can be used in conjunction with the direct PCR metho...

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Bibliographic Details
Published inForensic science international Vol. 267; pp. 210 - 217
Main Authors Kitpipit, Thitika, Penchart, Kitichaya, Ouithavon, Kanita, Satasook, Chutamas, Linacre, Adrian, Thanakiatkrai, Phuvadol
Format Journal Article
LanguageEnglish
Published Ireland Elsevier B.V 01.10.2016
Elsevier Limited
Subjects
Amplification
Animals
Assaying
Collapse
Conservation of Natural Resources
Crime
Deoxyribonucleic acid
DNA Fingerprinting
Elephant
Elephants
Elephants - genetics
Forensic engineering
Forensic sciences
Identification
Ivory
Melt curve
Melts
National parks
Pathology
Polymorphism, Single Nucleotide
Real time
Real time PCR
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Species Specificity
Transition Temperature
Wildlife conservation
Thailand
China
Melt curve
Identification
Real time PCR
Ivory
Elephant
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Summary:Highlights • The real-time PCR based assay was proposed for ivory species identification. • Three extant elephant species were clearly classified by the developed assay. • The assay was fully validated according to ISFG recommendation. • The assay can be used in conjunction with the direct PCR method.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2016.08.037