Effect of thioredoxin on the sensitivity of bacteria to chemical damage
The ability of thioredoxin (Trx) to protect cells from chemical damage was determined by comparing the growth of a control strain of Escherichia coli JM101 and isogenic strain transformed with the plasmid pKKTS1 containing the Streptomyces aureofacien thioredoxin gene, in the presence of the nucleos...
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Published in | Journal of basic microbiology Vol. 39; no. 2; pp. 137 - 140 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin
WILEY-VCH Verlag Berlin GmbH
01.01.1999
WILEY‐VCH Verlag Berlin GmbH |
Subjects | |
Online Access | Get full text |
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Summary: | The ability of thioredoxin (Trx) to protect cells from chemical damage was determined by comparing the growth of a control strain of Escherichia coli JM101 and isogenic strain transformed with the plasmid pKKTS1 containing the Streptomyces aureofacien thioredoxin gene, in the presence of the nucleoside analogs arabinosylcytosine, 5‐fluorouridine, ftorafur and carcinogen β‐naftylamine. Arabinosylcytosine showed no effect on the growth of either of the two strains. 5‐fluorouridine, ftorafur [1‐((R,S)‐tetrahydrofuran‐2‐yl)‐5‐fluorouracil] and β‐naftylamine demonstrated lower inhibitory effects on the growth of the thioredoxin overproducing strain than on the growth of the control strain. These results suggested that Trx could protect the cells from chemical damage under certain metabolic conditions. |
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Bibliography: | istex:56B0F9714121B24F0F731DCB8FEE5039BC2CB24F ArticleID:JOBM137 ark:/67375/WNG-DPZF982K-N |
ISSN: | 0233-111X 1521-4028 |
DOI: | 10.1002/(SICI)1521-4028(199905)39:2<137::AID-JOBM137>3.0.CO;2-M |