Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry

• Published in: Biomedical chromatography Vol. 27; no. 9; pp. 1188 - 1191
• Main Authors: Abdel Rehim, Abbi, Abdel Rehim, Mohamed
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.09.2013
• Subjects: Chromatography, Liquid - methods; eVol; Humans; LC-MS/MS; lidocaine; Lidocaine - analysis; Lidocaine - isolation & purification; MEPS; Models, Chemical; Pharmaceutical Preparations - analysis; Pharmaceutical Preparations - isolation & purification; Saliva - chemistry; saliva samples; Solid Phase Microextraction - methods; Tandem Mass Spectrometry - methods; LC-MS/MS; eVol; saliva samples; lidocaine; MEPS
• ISSN: 0269-3879; 1099-0801; 1099-0801
• DOI: 10.1002/bmc.2925

ABSTRACT This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8‐cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS‐LC‐MS/MS is reported. Copyright © 2013 John Wiley & Sons, Ltd.