Brain‐derived neurotrophic factor, corticotropin‐releasing factor, and hypothalamic neuronal histamine interact to regulate feeding behavior

• Published in: Journal of neurochemistry Vol. 125; no. 4; pp. 588 - 598
• Main Authors: Gotoh, Koro, Masaki, Takayuki, Chiba, Seiichi, Ando, Hisae, Fujiwara, Kansuke, Shimasaki, Takanobu, Mitsutomi, Kimihiko, Katsuragi, Isao, Kakuma, Tetsuya, Sakata, Toshiie, Yoshimatsu, Hironobu
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.05.2013
• Subjects: Animal behavior; Animals; Anorexia - chemically induced; Anorexia - physiopathology; BDNF; Body Weight - drug effects; Body Weight - physiology; Brain; Brain-derived neurotrophic factor; Brain-Derived Neurotrophic Factor - metabolism; Brain-Derived Neurotrophic Factor - pharmacology; Brain-Derived Neurotrophic Factor - physiology; Corticotropin-Releasing Hormone - physiology; CRH; Eating - drug effects; Eating - physiology; feeding behavior; Feeding Behavior - drug effects; Feeding Behavior - physiology; Histamine; Histamine - metabolism; Histamine - physiology; Hormones; hypothalamus; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Neurochemistry; Rats; Rats, Sprague-Dawley; Receptors, Histamine H1 - genetics; Receptors, Histamine H1 - metabolism; Rodents; Ventromedial Hypothalamic Nucleus - drug effects; Ventromedial Hypothalamic Nucleus - physiology
• ISSN: 0022-3042; 1471-4159
• DOI: 10.1111/jnc.12213

Brain‐derived neurotrophic factor (BDNF), corticotropin‐releasing factor (CRF), and hypothalamic neuronal histamine are anorexigenic substances within the hypothalamus. This study examined the interactions among BDNF, CRF, and histamine during the regulation of feeding behavior in rodents. Food intake was measured after treatment with BDNF, α‐fluoromethyl histidine (FMH; a specific suicide inhibitor of histidine decarboxylase that depletes hypothalamic neuronal histamine), or CRF antagonist. We measured food intake in wild‐type mice and mice with targeted disruption of the histamine H1 receptor (H1KO mice) after central BDNF infusion. Furthermore, we investigated CRF content and histamine turnover in the hypothalamus after BDNF treatment, and conversely, BDNF content in the hypothalamus after histamine treatment. We used immunohistochemical staining for histamine H1 receptors (H1‐R) in BDNF neurons. BDNF‐induced feeding suppression was partially attenuated in rats pre‐treated with FMH or a CRF antagonist, and in H1KO mice. BDNF treatment increased CRF content and histamine turnover in the hypothalamus. Histamine increased BDNF content in the hypothalamus. Immunohistochemical analysis revealed that H1‐Rs were expressed on BDNF neurons in the ventromedial nucleus of the hypothalamus. These results indicate that CRF and hypothalamic neuronal histamine mediate the suppressive effects of BDNF on feeding behavior and body weight. We have developed a working model of neuronal network via signaling between brain‐derived neurotrophic factor (BDNF) and neuronal histamine. BDNF may act on histamine neurons in in the tuberomammillary nucleus (TMN) through corticotropin‐releasing factor (CRF) neurons in the paraventricular nucleus (PVN). Furthermore, neuronal histamine also stimulates BDNF neurons in the ventromedial nucleus (VMH) directly.