Antiviral activity of porcine interferon omega 7 against foot‐and‐mouth disease virus in vitro

• Published in: Journal of medical virology Vol. 91; no. 2; pp. 208 - 214
• Main Authors: Li, Shi‐Fang, Zhao, Fu‐Rong, Gong, Mei‐Jiao, Shao, Jun‐Jun, Xie, Yin‐Li, Chang, Hui‐Yun, Zhang, Yong‐Guang
• Format: Journal Article Web Resource
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.02.2019; John Wiley & Sons; John Wiley and Sons Inc
• Subjects: Animals; Antiviral activity; Antiviral Agents - pharmacology; Cell proliferation; Chemical compounds; Cytopathogenic Effect, Viral; E coli; Economic importance; Foot & mouth disease; foot-and-mouth disease virus (FMDV; Foot-and-Mouth Disease Virus - drug effects; Foot-and-Mouth Disease Virus - growth & development; Fusion protein; Genes; Infections; Interferon; interferon stimulated genes (ISG); Interferon Type I - genetics; Interferon Type I - pharmacology; Life sciences; Livestock; Microbiologie; Microbiology; Myxovirus resistance proteins; nterferon stimulated genes (ISG); Outbreaks; Pharmacology; Polymerase chain reaction; porcine IFN omega 7 (PoIFN‐ω7); Proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - pharmacology; Sciences du vivant; Swine; Transcription; type I interferon; Vaccines; Virology; Viruses; type I interferon; porcine IFN omega 7 (PoIFN-ω7); antiviral activity; foot-and-mouth disease virus (FMDV); interferon stimulated genes (ISG)
• ISSN: 0146-6615; 1096-9071; 1096-9071
• DOI: 10.1002/jmv.25272

Foot‐and‐mouth disease (FMD) is a disease of worldwide economic importance, and vaccines play an important role in preventing FMDV outbreaks. However, new control strategies are still needed since FMDV outbreaks still occur in some disease‐free countries. Currently, interferon (IFN)‐based strategies have been demonstrated to be an efficient biotherapeutic option against FMDV; however, interferon omega (IFN‐ω) has not yet been assessed in this capacity. Thus, this study evaluated the antiviral activity of porcine IFN omega 7 (PoIFN‐ω7) against FMDV. After the PoIFN‐ω7 was expressed and purified, cell proliferation assays and quantitative real‐time reverse transciption‐polymerase chain reaction were used to evaluate the effective anti‐cytopathic concentration of PoIFN‐ω7 and its effectiveness pre‐ and post‐infection with FMDV in swine kidney cells (IBRS‐2). Results showed the rHis‐PoIFN‐ω7 fusion protein was considerably expressed using Escherichia coli BL21 (DE3) strain, and the recombinant protein exhibited significant in vitro protection against FMDV, including two strains belonging to type O and A FMDV, respectively. In addition, PoIFN‐ω7 upregulated the transcription of Mx1, ISG15, OAS1, and PKR genes. These findings indicated that IFN‐ω has the potential for serving as a useful therapeutic agent to prevent FMDV or other viral outbreaks in pigs. 1.PoIFN‐ω7 exerted effective antiviral activity against FMDV pre‐ and post‐infection in vitro. 2.PoIFN‐ω7 induced the transcription of IFN‐stimulated genes, including Mx1, ISG15, OAS1, and PKR genes.