3D structural analysis of protein O‐mannosyl kinase, POMK, a causative gene product of dystroglycanopathy

• Published in: Genes to cells : devoted to molecular & cellular mechanisms Vol. 22; no. 4; pp. 348 - 359
• Main Authors: Nagae, Masamichi, Mishra, Sushil K., Neyazaki, Makiko, Oi, Rika, Ikeda, Akemi, Matsugaki, Naohiro, Akashi, Satoko, Manya, Hiroshi, Mizuno, Mamoru, Yagi, Hirokazu, Kato, Koichi, Senda, Toshiya, Endo, Tamao, Nogi, Terukazu, Yamaguchi, Yoshiki
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.04.2017
• Subjects: Animals; Catalytic Domain; Crystallography, X-Ray; Dystroglycans - chemistry; Humans; Kinases; Mice; Muscular Dystrophies - genetics; Muscular Dystrophies - metabolism; Mutation; Protein Kinases - chemistry; Protein Kinases - genetics; Protein Kinases - metabolism; Proteins
• ISSN: 1356-9597; 1365-2443
• DOI: 10.1111/gtc.12480

Orchestration of the multiple enzymes engaged in O‐mannose glycan synthesis provides a matriglycan on α‐dystroglycan (α‐DG) which attracts extracellular matrix (ECM) proteins such as laminin. Aberrant O‐mannosylation of α‐DG leads to severe congenital muscular dystrophies due to detachment of ECM proteins from the basal membrane. Phosphorylation at C6‐position of O‐mannose catalyzed by protein O‐mannosyl kinase (POMK) is a crucial step in the biosynthetic pathway of O‐mannose glycan. Several mis‐sense mutations of the POMK catalytic domain are known to cause a severe congenital muscular dystrophy, Walker–Warburg syndrome. Due to the low sequence similarity with other typical kinases, structure–activity relationships of this enzyme remain unclear. Here, we report the crystal structures of the POMK catalytic domain in the absence and presence of an ATP analogue and O‐mannosylated glycopeptide. The POMK catalytic domain shows a typical protein kinase fold consisting of N‐ and C‐lobes. Mannose residue binds to POMK mainly via the hydroxyl group at C2‐position, differentiating from other monosaccharide residues. Intriguingly, the two amino acid residues K92 and D228, interacting with the triphosphate group of ATP, are donated from atypical positions in the primary structure. Mutations in this protein causing muscular dystrophies can now be rationalized. Phosphorylation of O‐mannose by protein O‐mannosyl kinase, POMK, is a crucial step in the biosynthetic pathway of O‐mannose glycan of α‐dystroglycan. Here we determined the crystal structure of murine POMK catalytic domain and identified the amino acid residues required for nucleotide binding, glycan recognition and catalysis. Our study will provide insights into POMK mutations found in a congenital muscular dystrophy, Walker–Warburg syndrome.