H2A.B facilitates transcription elongation at methylated CpG loci

H2A.B is a unique histone H2A variant that only exists in mammals. Here we found that H2A.B is ubiquitously expressed in major organs. Genome-wide analysis of H2A.B in mouse ES cells shows that H2A.B is associated with methylated DNA in gene body regions. Moreover, H2A.B-enriched gene loci are activ...

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Bibliographic Details
Published inGenome research Vol. 24; no. 4; pp. 570 - 579
Main Authors Chen, Yibin, Chen, Qiang, McEachin, Richard C, Cavalcoli, James D, Yu, Xiaochun
Format Journal Article
LanguageEnglish
Published United States Cold Spring Harbor Laboratory Press 01.04.2014
Subjects
Animals
CpG Islands
DNA Methylation - genetics
Gene Expression Regulation
Genetic Variation
Genome
Histones - biosynthesis
Histones - genetics
Mice
Promoter Regions, Genetic
Transcription, Genetic
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Summary:H2A.B is a unique histone H2A variant that only exists in mammals. Here we found that H2A.B is ubiquitously expressed in major organs. Genome-wide analysis of H2A.B in mouse ES cells shows that H2A.B is associated with methylated DNA in gene body regions. Moreover, H2A.B-enriched gene loci are actively transcribed. One typical example is that H2A.B is enriched in a set of differentially methylated regions at imprinted loci and facilitates transcription elongation. These results suggest that H2A.B positively regulates transcription elongation by overcoming DNA methylation in the transcribed region. It provides a novel mechanism by which transcription is regulated at DNA hypermethylated regions.
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ISSN:1088-9051
1549-5469
DOI:10.1101/gr.156877.113