Characterization of a Lectin from the Leaves of Great Northern Bean, Phaseolus vulgaris L

• Published in: Bioscience, biotechnology, and biochemistry Vol. 60; no. 4; pp. 608 - 611
• Main Authors: Kamemura, Kazuo, Ozeki, Munetaka, Furuichi, Yukio, Umekawa, Hayato, Takahashi, Takao
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 1996; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Binding and carrier proteins; Biological and medical sciences; Biotechnology; Cross Reactions; Fundamental and applied biological sciences. Psychology; Great Northern bean; Horseradish Peroxidase; leaf; Molecular Sequence Data; non-seed lectin; Phaseolus vulgaris; Phytohemagglutinins - analysis; Plant Leaves - chemistry; Plant Lectins; Protein Binding; Proteins; Sequence Homology, Amino Acid; Lectin; Composition; Purification; Ligand; Oligosaccharide; Molecular interaction; Plant leaf; Subunit; Characterization; Grain legume; Specificity; Leguminosae; Phaseolus vulgaris; Dicotyledones; Aminoacid; Angiospermae; N terminal-Sequence; Spermatophyta; Structural analysis
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.60.608

A novel lectin (GN L L) was isolated from the leaves of the Great Northern bean, Phaseolus vulgaris. GN L L was purified by affinity chromatography on ovomucoid-Sepharose 4B. GN L L had a molecular mass of 135 kDa on gel filtration and gave two bands on SDS-polyacrylamide gel electrophoresis (PAGE) (band A of 34.0 kDa and band B of 34.2 kDa). Binding assay of horseradish peroxidase (HRP)-glycoproteins to the bands electroblotted onto polyvinylidene difluoride (PVDF) membrane showed that both bands could bind to complex-type N-linked oligosaccharide chains in glycoproteins. The N-terminal amino acid sequences of both bands were identical through the 10 residues and identical to that of α-subunit of a pod lectin (pod-α-subunit) from the same bean. On the other hand, band B cross-reacted with monoclonal antibody against a seed lectin from the same bean, but band A did not.