Effects of interleukin-4 on human osteoblast-like cells

• Published in: Bone and mineral Vol. 21; no. 1; pp. 53 - 61
• Main Authors: Riancho, J.A., Zarrabeitia, M.T., Olmos, J.M., Amado, J.A., Gonzalez-Macias, J.
• Format: Journal Article
• Language: English
• Published: London Elsevier Inc 01.04.1993; Amsterdam Elsevier; New York, NY
• Subjects: 1,25(OH) 2D 3; 1,25-dihydroxyvitamin D 3; Alkaline phosphatase; Alkaline Phosphatase - metabolism; Biological and medical sciences; Calcitriol - pharmacology; Cell Division - drug effects; Cell Line; Cell physiology; DNA - biosynthesis; Enzyme-Linked Immunosorbent Assay; FCS; fetal calf serum; Fundamental and applied biological sciences. Psychology; Humans; interleukin; Interleukin-4 - pharmacology; Interleukin-6 - metabolism; Interleukins; lipopolysaccharide; LPS; Molecular and cellular biology; Osteoblasts; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - metabolism; p-nitrophenol; PNP; Responses to growth factors, tumor promotors, other factors; TNF; tumor necrosis factor; Tumor Necrosis Factor-alpha - metabolism; Alkaline phosphatase; IL; p-nitrophenol; 1,25(OH) 2D 3; Osteoblasts; TNF; LPS; PNP; Interleukins; tumor necrosis factor; 1,25-dihydroxyvitamin D 3; FCS; fetal calf serum; interleukin; lipopolysaccharide; Human; Cell culture; Enzyme; Growth; Cytokine; Osteoarticular system; Interleukin 4; Enzymatic activity; Established cell line; Osteoblast; Differentiation; Morphometry
• ISSN: 0169-6009
• DOI: 10.1016/S0169-6009(08)80120-1

Interleukin-4 (IL-4) modulates the activity of a variety of lymphoid, hemopoietic and mesenchymal cells, but little is known about its influence on bone cells. We have studied the effects of IL-4 on the human osteoblast-like cell line MG63. IL-4 (0.1-50 ng/ml) inhibited cell proliferation. The effect did not depend on cell density, but it was more marked in serum-free cultures than in the presence of serum. IL-4 also induced a dose-dependent increase in the expression of alkaline phosphatase stimulated by 1,25dihydroxyvitamin D3, a marker of differentiated osteoblast activity. However, IL-4 did not modify the secretion of interleukin-6 or tumor necrosis factor. These results suggest that interleukin-4 may play a role as a modulator of osteoblast activity.