Effects of maternal exposure to lead on secondary sex ratio in Japan: The Japan Environment and Children’s Study

• Published in: The Science of the total environment Vol. 817; p. 152726
• Main Authors: Tatsuta, Nozomi, Nakai, Kunihiko, Nakayama, Shoji F., Takeuchi, Ayano, Arima, Takahiro, Yaegashi, Nobuo, Kamijima, Michihiro, Yamazaki, Shin, Ohya, Yukihiro, Kishi, Reiko, Hashimoto, Koichi, Mori, Chisato, Ito, Shuichi, Yamagata, Zentaro, Inadera, Hidekuni, Nakayama, Takeo, Iso, Hiroyasu, Shima, Masayuki, Kurozawa, Youichi, Suganuma, Narufumi, Kusuhara, Koichi, Katoh, Takahiko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.04.2022
• Subjects: Air lead level; Blood lead level; Child; Cohort Studies; Female; Humans; Japan; Lead; Male; Maternal Exposure; Pregnancy; Prenatal exposure; Secondary sex ratio; Sex Ratio; Japan; ALL; BLL; JECS; K6; DAG; PCB; Prenatal exposure; Air lead level; SSR; Lead; Secondary sex ratio; ICP-MS; Blood lead level
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2021.152726

A decline in the proportion of male births (secondary sex ratio, SSR) has been seen in several countries including Japan in recent years. Although previous studies have reported that the SSR is affected by exposure to chemical substances such as dioxins and polychlorinated biphenyls, as well as heavy metals such as methylmercury, the effects of lead exposure on the SSR have been little studied. The aim of this study was to determine the association between maternal lead exposure and SSR. In a large-scale nationwide birth cohort study, maternal blood lead level (BLL) was determined using whole blood from the second or third trimester of pregnancy. The association between SSR and maternal BLL was estimated using multivariable logistic models. Binomial distribution was applied to examine the differences in SSR by dividing the participants into five groups based on BLL. The primary outcome was SSR, and the child sex was obtained from the medical record transcripts. Of 104,062 fetal records, 85,171 were examined for analysis. The median maternal BLL was 5.85 ng/g (5th–95th percentile 3.45–10.6 ng/g). The overall proportion of males among participating infants was 0.512. In logistic regression models adjusted for covariates, the analysis revealed an increased odds ratio for SSR with higher blood lead concentrations [Group 2: adjusted OR 1.082, 95% confidence interval 1.037 to 1.129, Group 3: 1.122, 1.074 to 1.171, Group 4: 1.214, 1.163 to 1.268, Group 5: 1.279, 1.224 to 1.336]. Compared to the general birth probability in Japan, the group with low BLL had a lower SSR and the group with high BLL had a higher SSR. Higher maternal lead exposures during pregnancy were associated with increased SSR. Further investigations including assessment of paternal lead exposure are necessary to understand the association between lead exposure and SSR. [Display omitted] •The association between blood lead levels in second/third trimester and secondary sex ratio was examined.•The higher the blood lead level, the higher the secondary sex ratio.•Lead exposure increases the rate at which males are born.•Blood lead level decreases due to the decrease in air lead level, secondary sex ratio may also be linked.