Identification and Analysis of GhEXO Gene Family Indicated That GhEXO7_At Promotes Plant Growth and Development Through Brassinosteroid Signaling in Cotton (Gossypium hirsutum L.)
Brassinosteroids (BRs), an efficient plant endogenous hormone, significantly promotes plant nutrient growth adapting to biological and abiotic adversities. BRs mainly promote plant cell elongation by regulating gene expression patterns. EXORDIUM ( EXO ) genes have been characterized as the indicator...
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Published in | Frontiers in plant science Vol. 12; p. 719889 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
16.09.2021
|
Subjects | |
Online Access | Get full text |
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Summary: | Brassinosteroids (BRs), an efficient plant endogenous hormone, significantly promotes plant nutrient growth adapting to biological and abiotic adversities. BRs mainly promote plant cell elongation by regulating gene expression patterns.
EXORDIUM
(
EXO
) genes have been characterized as the indicators of BR response genes. Cotton, an ancient crop, is of great economic value and its fibers can be made into all kinds of fabrics. However,
EXO
gene family genes have not been full identified in cotton. 175
EXO
genes were identified in nine plant species, of which 39
GhEXO
genes in
Gossypium hirsutum
in our study. A phylogenetic analysis grouped all of the proteins encoded by the
EXO
genes into five major clades. Sequence identification of conserved amino acid residues among monocotyledonous and dicotyledonous species showed a high level of conservation across the N and C terminal regions. Only 25% the
GhEXO
genes contain introns besides conserved gene structure and protein motifs distribution. The 39
GhEXO
genes were unevenly distributed on the 18 At and Dt sub-genome chromosomes. Most of the
GhEXO
genes were derived from gene duplication events, while only three genes showed evidence of tandem duplication. Homologous locus relationships showed that 15
GhEXO
genes are located on collinear blocks and that all orthologous/paralogous gene pairs had
Ka
>
Ks
values, indicating purifying selection pressure. The
GhEXO
genes showed ubiquitous expression in all eight tested cotton tissues and following exposure to three phytohormones, IAA, GA, and BL. Furthermore,
GhEXO7_At
was mainly expressed in response to BL treatment, and was predominantly expressed in the fibers.
GhEXO7_At
was found to be a plasma membrane protein, and its ectopic expression in
Arabidopsis
mediated BR-regulated plant growth and development with altered expression of
DWF4
,
CPD
,
KCS1
, and
EXP5
. Additionally, the functions of
GhEXO7_At
were confirmed by virus-induced gene silencing (VIGS) in cotton. This study will provide important genetic resources for future cotton breeding programs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Honghong Wu, Huazhong Agricultural University, China These authors have contributed equally to this work Reviewed by: Xianchen Zhang, Anhui Agricultural University, China; Lan Zhu, Huazhong Agricultural University, China This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2021.719889 |