Highly sensitive and selective derivatization-LC method for biomolecules based on fluorescence interactions and fluorous separations

A fluorescence derivatization LC method is a powerful tool for the analysis with high sensitivity and selectivity of biological compounds. In this review, we introduce new types of fluorescence derivatization LC analysis methods. These are (1) detection-selective derivatization methods based on fluo...

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Bibliographic Details
Published inJournal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 879; no. 17; pp. 1325 - 1337
Main Authors Todoroki, Kenichiro, Yoshida, Hideyuki, Hayama, Tadashi, Itoyama, Miki, Nohta, Hitoshi, Yamaguchi, Masatoshi
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.05.2011
Subjects
Amino Acids - chemistry
Animals
biochemical compounds
Biomolecules
Carboxylic Acids - chemistry
Chromatography
Chromatography, Liquid - methods
derivatization
Energy transfer
Excimer fluorescence
F-trap fluorescence derivatization
Flavonoids - chemistry
Fluorescence
Fluorescence derivatization
Fluorescence Resonance Energy Transfer - methods
Fluorescent Dyes - chemistry
Fluorous derivatization
Fluorous scavenging derivatization
FRET
Fretting
Humans
Phenols - chemistry
Polyamines - chemistry
Polyphenols
Scavenging
Separation
Spectrometry, Fluorescence - methods
Sulfhydryl Compounds - chemistry
Excimer fluorescence
FRET
Fluorescence derivatization
Fluorous derivatization
F-trap fluorescence derivatization
Fluorous scavenging derivatization
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Summary:A fluorescence derivatization LC method is a powerful tool for the analysis with high sensitivity and selectivity of biological compounds. In this review, we introduce new types of fluorescence derivatization LC analysis methods. These are (1) detection-selective derivatization methods based on fluorescence interactions generated from fluorescently labeled analytes: excimer fluorescence derivatization and fluorescence resonance energy transfer (FRET) derivatization; (2) separation-selective derivatization methods using the fluorous separation technique: fluorous derivatization, F-trap fluorescence derivatization, and fluorous scavenging derivatization (FSD).
Bibliography:http://dx.doi.org/10.1016/j.jchromb.2010.11.038
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ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2010.11.038