Marinobacterium maritimum sp. nov., a marine bacterium isolated from Arctic sediment

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 12; pp. 3030 - 3034
• Main Authors: Kim, So-Jeong, Park, Soo-Je, Yoon, Dae-No, Park, Byoung-Joon, Choi, Bo-Ram, Lee, Dong-Hun, Roh, Yul, Rhee, Sung-Keun
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.12.2009; Society for General Microbiology
• Subjects: Alteromonadaceae - classification; Alteromonadaceae - genetics; Alteromonadaceae - isolation & purification; Alteromonadaceae - metabolism; Arctic Regions; Bacteria; Bacteriology; Biological and medical sciences; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fatty Acids - chemistry; Fatty Acids - metabolism; Fundamental and applied biological sciences. Psychology; Geologic Sediments - microbiology; Marinobacterium; Marinobacterium georgiense; Microbiology; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Systematics; Arctic Region; Arctic Regions; PN, Arctic; Marine environment; Bacteria; Marine sediments; New species; Taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.009134-0

1 Department of Microbiology, Chungbuk National University, 12 Gaeshin-dong, Cheongju 361-763, Republic of Korea 2 Faculty of Earth Systems and Environmental Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea Correspondence Sung-Keun Rhee rhees{at}chungbuk.ac.kr A Gram-negative, aerobic, rod-shaped, motile, marine bacterium, strain AR11 T , was isolated from Arctic marine sediment. Strain AR11 T grew with 0.5–7 % NaCl and at 7–37 °C and pH 5.5–9.0. It utilized propionate, 3-hydroxybenzoate, L -proline, acetate, D - and L -lactate, L -alanine, malate and phenylacetic acid. Alkaline phosphatase, esterase lipase (C8), leucine arylamidase and acid phosphatase activity tests were positive. Acid was produced from 5-ketogluconate and aesculin. Strain AR11 T possessed C 16 : 0 (22.0 %), summed feature 4 (C 16 : 1 7 c and/or iso-C 15 : 0 2-OH; 28.1 %) and summed feature 7 (one or more of C 18 : 1 7 c , 9 t and 12 t ; 34.0 %) as the major cellular fatty acids. The major ubiquinone was Q-8. Comparative 16S rRNA gene sequence studies showed that strain AR11 T belonged to the Gammaproteobacteria and was most closely related to Marinobacterium stanieri DSM 7027 T , Marinobacterium halophilum mano11 T and Marinobacterium georgiense KW-40 T (97.8, 97.0 and 96.7 % similarity, respectively). The G+C content of the genomic DNA of strain AR11 T was 57.9 mol%. DNA–DNA relatedness data indicated that strain AR11 T represented a distinct species that was separated from M. stanieri DSM 7027 T , M. halophilum KCTC 12240 T and M. georgiense JCM 21667 T . On the basis of evidence from this polyphasic study, it is proposed that strain AR11 T (=KCTC 22254 T =JCM 15134 T ) represents the type strain of a novel species, Marinobacterium maritimum sp. nov. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain AR11 T is EU399548 . A maximum-likelihood tree based on 16S rRNA gene sequences is available as supplementary material with the online version of this paper.