The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by Rhizobium leguminosarum

• Published in: Biochimica et biophysica acta Vol. 539; no. 1; pp. 1 - 11
• Main Authors: Bisseling, T., Van Den Bos, R.C., Van Kammen, A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 13.02.1978
• Subjects: biosynthesis; drug effects; Enzyme Precursors; Enzyme Precursors - biosynthesis; Fabaceae; Fabaceae - metabolism; Hemeproteins; Hemeproteins - metabolism; horticultural crops; Leghemoglobin; Leghemoglobin - metabolism; metabolism; Nitrates; Nitrates - pharmacology; Nitrogen Fixation; Nitrogen Fixation - drug effects; Nitrogenase; Nitrogenase - biosynthesis; pharmacology; plant biochemistry; plant physiology; Plants, Medicinal; Quaternary Ammonium Compounds; Quaternary Ammonium Compounds - pharmacology; Rhizobium; Rhizobium - metabolism; Symbiosis; SDS
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(78)90115-0

The effects of NH 4NO 3 on the development of root nodules of Pisum sativum after infection with Rhizobium leguminosarum (strain PRE) and on the nitrogenase activity of the bacteriods in the nodule tissue were studied. The addition of NH 4NO 3 decreased the nitrogenase activity measured on intact nodules. This reduction of nitrogen fixation did not result from a reduced number of bacteroids or a decreased amount of bacteroid proteins per gram of nodule. The synthesis of nitrogenase, measured as the relative amount of incorporation of [ 35S]sulfate into the components I and II of nitrogenase was similarly not affected. The addition of NH 4NO 3 decreased the amount of leghemoglobin in the nodules and there was a quantitative correlation between the leghemoglobin content and the nitrogen-fixing capacity of the nodules. The conclusion is that the decrease of nitrogen-fixing capacity is caused by a decrease of the leghemoglobin content of the root nodules and not by repression of the nitrogenase synthesis.