An optimized protocol of a human-to-cattle interspecies somatic cell nuclear transfer

Human somatic cells were transferred into cattle enucleated oocytes, and a prospective, randomized study was designed to optimize donor cell preparation, fusion medium, and culture method. As a result, improved development of interspecies embryos was achieved by employing serum-starved cord fibrobla...

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Published inFertility and sterility Vol. 82; no. 4; pp. 960 - 962
Main Authors Chang, Kyung H., Lim, Jeong M., Kang, Sung K., Lee, Byeong C., Moon, Shin Y., Hwang, Woo S.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.10.2004
Subjects
Animals
Cattle - embryology
Cell Culture Techniques
Cell Fusion - methods
Culture Media
Female
Fibroblasts - cytology
Gene Transfer, Horizontal - genetics
Humans
Mammary Glands, Human - cytology
Oocytes - cytology
Prospective Studies
Random Allocation
Umbilical Cord - cytology
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Summary:Human somatic cells were transferred into cattle enucleated oocytes, and a prospective, randomized study was designed to optimize donor cell preparation, fusion medium, and culture method. As a result, improved development of interspecies embryos was achieved by employing serum-starved cord fibroblasts, with Ca 2+/Mg 2+-free fusion medium and a serum-free medium being used for bovine embryos.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2004.03.052