Regulation of feeding behavior and food intake by appetite-regulating peptides in wild-type and growth hormone-transgenic coho salmon

• Published in: Hormones and behavior Vol. 84; pp. 18 - 28
• Main Authors: White, Samantha L., Volkoff, Helene, Devlin, Robert H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2016; Elsevier BV
• Subjects: alpha-MSH - pharmacology; Animals; Animals, Genetically Modified; Anorexigenic peptide; Appetite; Appetite - drug effects; Appetite - physiology; Bombesin - pharmacology; Cholecystokinin - pharmacology; Coho salmon; Eating - drug effects; Eating - physiology; Feeding Behavior - drug effects; Feeding Behavior - physiology; Food intake; Foraging behavior; Genotype; Glucagon-Like Peptide 1 - pharmacology; Growth hormone; Growth Hormone - genetics; Hormones; Intraperitoneal injection; Oncorhynchus kisutch; Oncorhynchus kisutch - genetics; Orexigenic peptide; Salmon; Seasons; Transgenic; Transgenic animals; Orexigenic peptide; Food intake; Anorexigenic peptide; Oncorhynchus kisutch; Transgenic; Coho salmon; Growth hormone; Intraperitoneal injection
• ISSN: 0018-506X; 1095-6867
• DOI: 10.1016/j.yhbeh.2016.04.005

Survival, competition, growth and reproductive success in fishes are highly dependent on food intake, food availability and feeding behavior and are all influenced by a complex set of metabolic and neuroendocrine mechanisms. Overexpression of growth hormone (GH) in transgenic fish can result in greatly enhanced growth rates, feed conversion, feeding motivation and food intake. The objectives of this study were to compare seasonal feeding behavior of non-transgenic wild-type (NT) and GH-transgenic (T) coho salmon (Oncorhynchus kisutch), and to examine the effects of intraperitoneal injections of the appetite-regulating peptides cholecystokinin (CCK-8), bombesin (BBS), glucagon-like peptide-1 (GLP-1), and alpha-melanocyte-stimulating hormone (α-MSH) on feeding behavior. T salmon fed consistently across all seasons, whereas NT dramatically reduced their food intake in winter, indicating the seasonal regulation of appetite can be altered by overexpression of GH in T fish. Intraperitoneal injections of CCK-8 and BBS caused a significant and rapid decrease in food intake for both genotypes. Treatment with either GLP-1 or α-MSH resulted in a significant suppression of food intake for NT but had no effect in T coho salmon. The differential response of T and NT fish to α-MSH is consistent with the melanocortin-4 receptor system being a significant pathway by which GH acts to stimulate appetite. Taken together, these results suggest that chronically increased levels of GH alter feeding regulatory pathways to different extents for individual peptides, and that altered feeding behavior in transgenic coho salmon may arise, in part, from changes in sensitivity to peripheral appetite-regulating signals. •Appetite regulation was examined in wild-type and growth hormone transgenic salmon.•High levels of growth hormone in transgenic fish alter seasonal feeding.•Cholecystokinin and bombesin had suppressive effects on feeding in both genotypes.•Glucagon-like peptide-1 suppressed feeding in wild-type but not transgenic fish.•Alpha-melanocyte-stimulating hormone had no effect on food intake in transgenic fish.