Stereoselective blood-brain barrier transport of histidine in rats

• Published in: Brain research Vol. 812; no. 1; pp. 105 - 112
• Main Authors: Yamakami, Jun, Sakurai, Eiichi, Sakurada, Tomoya, Maeda, Kazumi, Hikichi, Noboru
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 23.11.1998; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Biological and medical sciences; Blood-brain barrier; Blood-Brain Barrier - physiology; Cells, Cultured; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges; Endothelium, Vascular - cytology; Endothelium, Vascular - physiology; Fundamental and applied biological sciences. Psychology; Histidine; Histidine - pharmacokinetics; Male; Rat; Rats; Rats, Wistar; Stereoisomerism; Stereoselective transport; Vertebrates: nervous system and sense organs; Stereoselective transport; Histidine; Blood-brain barrier; Rat; Stereoselectivity; Vertebrata; Mammalia; Biological transport; Rodentia; Central nervous system; Blood brain barrier; Brain (vertebrata)
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(98)00958-5

The transport characteristics of l- and d-histidine through the blood-brain barrier (BBB) were studied using cultured rat brain microvascular endothelial cells (BMEC). l-Histidine uptake was a saturable process. A decrease in incubation temperature from 37 to 0°C or the addition of metabolic inhibitors (DNP and rotenone) reduced the uptake rate of l-histidine. Ouabain, an inhibitor of (Na +, K +)-ATPase, also reduced uptake of l-histidine. Moreover, the substitution of Na + with choline chloride and choline bicarbonate in the incubation buffer decreased the initial l- and d-histidine uptake rates. These results suggested that l-histidine is actively uptaken by a carrier-mediated mechanism into the BMEC, with energy supplied by Na +. However, l-histidine uptake at 0°C was not completely inhibited, and it was reduced in the presence of an Na +-independent System-L substrate, BCH, suggesting facilitated diffusion (the Na +-independent process) by a carrier-mediated mechanism into the BMEC. l-histidine uptake in rat BMEC also appeared to be System-N mediated since uptake was inhibited by glutamine, aspargine and l-glutamic acid γ-monohydroxamate. System-N mediated transport was not pH sensitive. d-histidine transport was also studied in rat BMEC. d-histidine transport by rat BMEC has similar characteristics to l-histidine. However, System-N transport did not play a role in d-histidine uptake. The uptake of l-histidine was also greater than that of the d-isomer, indicating the stereoselective uptake of histidine in rat BMEC.