Preclinical evaluation and validation of [18F]HX4, a promising hypoxia marker for PET imaging
Hypoxia has been shown to be an important microenvironmental parameter influencing tumor progression and treatment efficacy. Patient guidance for hypoxia-targeted therapy requires evaluation of tumor oxygenation, preferably in a noninvasive manner. The aim of this study was to evaluate and validate...
Saved in:
Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 35; pp. 14620 - 14625 |
---|---|
Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
30.08.2011
National Acad Sciences |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Hypoxia has been shown to be an important microenvironmental parameter influencing tumor progression and treatment efficacy. Patient guidance for hypoxia-targeted therapy requires evaluation of tumor oxygenation, preferably in a noninvasive manner. The aim of this study was to evaluate and validate the uptake of [18F]HX4, a novel developed hypoxia marker for PET imaging. A heterogeneous accumulation of [18F]HX4 was found within rat rhabdomyosarcoma tumors that was significantly (P < 0.0001) higher compared with the surrounding tissues, with temporal increasing tumor-to-blood ratios reaching a plateau of 7.638 ± 0.926 and optimal imaging properties 4 h after injection. [18F]HX4 retention in normal tissues was found to be short-lived, homogeneous and characterized by a fast progressive temporal clearance. Heterogeneity in [18F]HX4 tumor uptake was analyzed based on 16 regions within the tumor according to the different orthogonal planes at the largest diameter. Validation of heterogeneous [18F]HX4 tumor uptake was shown by a strong and significant relationship (r = 0.722; P < 0.0001) with the hypoxic fraction as calculated by the percentage pimonidazole-positive pixels. Furthermore, a causal relationship with tumor oxygenation was established, because combination treatment of nicotinamide and carbogen resulted in a 40% reduction (P < 0.001) in [18F]HX4 tumor accumulation whereas treatment with 7% oxygen breathing resulted in a 30% increased uptake (P < 0.05). [18F]HX4 is therefore a promising candidate for noninvasive detection and evaluation of tumor hypoxia at a macroscopic level. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by Michael E. Phelps, University of California, Los Angeles, CA, and approved July 21, 2011 (received for review February 16, 2011) Author contributions: L.J.D., M.C.Ö., J.B., A.v.d.K., and P.L. designed research; L.J.D., N.G.L., M.H.M.J., W.J.M.P., A.D.W., and J.C.W. performed research; A.D.W., J.C.W., H.C.K., M.C.Ö., J.B., G.A.M.S.v.D., and A.v.d.K. contributed new reagents/analytic tools; L.J.D., N.G.L., M.H.M.J., W.J.M.P., J.C.W., J.B., and A.v.d.K. analyzed data; and L.J.D., N.G.L., M.H.M.J., and P.L. wrote the paper. |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.1102526108 |