Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography
High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane–ethanol–water (4:3:1, v/v). Using the above solvent system, preparative...
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Published in | Journal of Chromatography A Vol. 905; no. 1; pp. 151 - 155 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
05.01.2001
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of
n-hexane–ethanol–water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-9673 |
DOI: | 10.1016/S0021-9673(00)00987-0 |