Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography

High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane–ethanol–water (4:3:1, v/v). Using the above solvent system, preparative...

Full description

Saved in:
Bibliographic Details
Published inJournal of Chromatography A Vol. 905; no. 1; pp. 151 - 155
Main Authors Li, Hua-Bin, Chen, Feng, Zhang, Tian-You, Yang, Fu-Quan, Xu, Guo-Qing
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 05.01.2001
Elsevier
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carotenoids
Chlorella - chemistry
Chlorella vulgaris
Chromatography, High Pressure Liquid - methods
Fundamental and applied biological sciences. Psychology
Lutein
Lutein - isolation & purification
Other biological molecules
Spectrophotometry, Ultraviolet
Terpenes, steroids. Hormones
Vitamins
Lutein
Carotenoids
Counter-current chromatography
Chlorella vulgaris
Preparative chromatography
Vitamins
Countercurrent chromatography
Purification
Phase composition
Algae
Chlorophyceae
Separation method
Chlorophyta
Plant origin
Carotenoid
Thallophyta
Online AccessGet full text

Cover

More Information
Summary:High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane–ethanol–water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)00987-0