Discrimination between different types of human adeno-associated viruses in clinical samples by PCR

• Published in: Journal of virological methods Vol. 71; no. 1; pp. 17 - 25
• Main Authors: Tobiasch, Edda, Burguete, Tatiana, Klein-Bauernschmitt, Petra, Heilbronn, Regine, Schlehofer, Jörg R.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.03.1998; Amsterdam Elsevier; New York, NY
• Subjects: AAV serotypes; Adeno-associated virus; Base Sequence; Biological and medical sciences; Dependovirus - classification; Dependovirus - genetics; Dependovirus - isolation & purification; DNA Primers - genetics; DNA, Viral - genetics; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; HeLa Cells; Humans; Microbiology; Molecular Sequence Data; PCR; Polymerase Chain Reaction - methods; Sequence Alignment; Sequence Analysis; Sequence Analysis, DNA - methods; Serotyping; Techniques used in virology; Virology; Virus detection; Adeno-associated virus; AAV serotypes; Virus detection; PCR; Virus; Polymerase chain reaction; Type specificity; Parvoviridae; Method; Dependovirus; Detection; Parvovirinae
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(97)00198-5

Persistent infection of human tissues with the helper virus-dependent parvovirus, adeno-associated virus (AAV) was detected by polymerase chain reaction (PCR) using primer pairs detecting AAV types 2, 3 or 5. In order to develop PCR protocols which discriminate between the different serotypes of AAV, the DNA of AAV-5 was sequenced partially and compared with the published sequences of AAV-2 and -3. Type specific oligonucleotides and specific probes which allow the distinction between human AAV types by PCR are described.