Nocardia acidivorans sp. nov., isolated from soil of the island of Stromboli

• Published in: International journal of systematic and evolutionary microbiology Vol. 57; no. 6; pp. 1183 - 1187
• Main Authors: Kampfer, Peter, Huber, Birgit, Buczolits, Sandra, Thummes, Kathrin, Grun-Wollny, Iris, Busse, Hans-Jurgen
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.06.2007; Society for General Microbiology
• Subjects: Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Italy; Lipids - analysis; Microbiology; Molecular Sequence Data; Nocardia - chemistry; Nocardia - classification; Nocardia - genetics; Nocardia - isolation & purification; Nocardia acidivorans; Nocardia nova; Nocardia pseudobrasiliensis; Nucleic Acid Hybridization; Phylogeny; Quinones - analysis; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Soil Microbiology; Spores, Bacterial; Systematics; Italy; Europe; Actinomycetales; Soils; Nocardia; Taxonomy; Bacteria; Nocardiaceae; Actinomycetes; New species; Stromboli
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.64813-0

1 Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany 2 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria 3 Labor Grün-Wollny, D-35394 Giessen, Germany Correspondence Peter Kämpfer peter.kaempfer{at}agrar.uni-giessen.de A Gram-positive, non-spore-forming bacterium (strain GW4-1778 T ) was isolated from soil of the Italian island of Stromboli. 16S rRNA gene sequence similarity studies showed that strain GW4-1778 T is a member of the genus Nocardia , most closely related to Nocardia pseudobrasiliensis (GenBank accession no. DQ659914 ; 98.6 %), Nocardia nova (Z36930 ; 98.6 %), Nocardia niigatensis (AB092563 ; 98.4 %), Nocardia jiangxiensis (AY639902 ; 98.0 %), Nocardia uniformis (Z46752 ; 98.0 %) and Nocardia miyunensis (AY639901 ; 97.8 %). Strain GW4-1778 T could be distinguished from any other established Nocardia species by sequence similarity values of less than 97.5 %. Strain GW4-1778 T exhibited a quinone system with the predominant compound MK-8 (H 4 , -cycl) (99.5 %) and traces of MK-8 (H 4 ), characteristic for the genus Nocardia . The polar lipid profile of strain GW4-1778 T consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, a unknown polar lipid and trace amounts of two unknown lipids and the major fatty acids were C 15 : 0 , C 16 : 0 , C 17 : 1 8 c and 10-methyl C 17 : 0 . The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW4-1778 T from related species with 16S rRNA gene similarities of >97.5 %. Therefore, strain GW4-1778 T merits species status, for which the name Nocardia acidivorans sp. nov. is proposed, with the type strain GW4-1778 T (=CCUG 53410 T =CIP 109315 T =DSM 45049 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain GW4-1778 T is AM402972 . An extended neighbour-joining tree based on 16S rRNA gene sequences showing the positions of strain GW4-1778 T and other Nocardia species is available as supplementary material with the online version of this paper.