Concentration of viruses from environmental waters using nanoalumina fiber filters

• Published in: Journal of microbiological methods Vol. 81; no. 1; pp. 33 - 38
• Main Authors: Li, Dan, Shi, Han-chang, Jiang, Sunny C.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.2010; Elsevier
• Subjects: Adenoviridae; Adenoviridae - isolation & purification; Adenovirus; Aluminum Oxide; Biological and medical sciences; Filtration - methods; Fluorescence-Activated Cell Sorting; Fundamental and applied biological sciences. Psychology; Humans; Hydrogen-Ion Concentration; Microbiology; Nanoalumina filter; Quantitative PCR; Static Electricity; Techniques used in virology; Viral concentration; Viral Load; Virology; Viruses - isolation & purification; Water Microbiology; Nanoalumina filter; Viral concentration; Adenovirus; Fluorescence-Activated Cell Sorting; Quantitative PCR; Virus; Polymerase chain reaction; Adenoviridae; Environment; Quantitative analysis
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2010.01.018

Human viral contamination in drinking and recreational water may persist for extensive periods of time and cause a significant health risk concern. The aim of this study is to evaluate a viral recovery method using a new electropositive charged nanoalumina filter and to compare results with the widely used negatively charged HAWP filter by Millipore Inc. The recovery of infectious recombinant adenovirus type 5 (rAd5) was tested using the Fluorescence-Activated Cell Sorting (FACS) assay, in parallel with viral genomes recovery assay by quantitative PCR (qPCR). The mean infectivity recoveries were 82–91% by nanoalumina filters eluted with 3% beef extract (BE, pH6.0), and 78–90% by HAWP filters eluted with 3% BE (pH 9.0), respectively, from 1 L of environmental samples seeded with 1pfu/mL rAd5. The mean genome recoveries were 16–35% by nanoalumina filters eluted with BE (pH 6.0), and 29–66% by HAWP filters eluted with NaOH (pH 10.8) from different types of water, respectively. Water quality, concentration of viruses, filters, and elution buffers are factors that determine the viral recovery efficiencies. The nanoalumina filters also had higher filtration rates than HAWP filters for large volumes of environmental water samples (up to10 L), thus, have an advantage in concentrating infectious viruses from environments without pre-filtration, adjusting pH or adding multivalent cations.