Kinetic and functional analysis of the heparin-binding domain of fibronectin

• Published in: Biotechnology letters Vol. 30; no. 1; pp. 55 - 59
• Main Authors: Kang, Wonmo, Park, Sunok, Jang, Jun-Hyeog
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.01.2008; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Adhesion; Applied Microbiology; Binding sites; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cell Adhesion; Cell adhesion & migration; Cell Line, Tumor; Cell Movement; Cellular biology; extracellular matrix; Fibronectin; Fibronectins - chemistry; Fibronectins - metabolism; Fundamental and applied biological sciences. Psychology; heparin; Heparin - chemistry; Heparin - metabolism; Humans; Kinetics; Life Sciences; Metabolic Clearance Rate; Microbiology; Original Research Paper; Osteoblast; Osteosarcoma - metabolism; Protein Binding; Protein Structure, Tertiary; Osteoblast; Extracellular matrix; Heparin; Fibronectin; Cell adhesion; Functional analysis; Kinetics
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-007-9522-3

We have previously shown that the heparin-binding domain of fibronectin (FN-HBD) enhances cell adhesion and proliferation of osteoblasts. Here we demonstrated that FN-HBD binds to heparin with a KD of 5 μM. Although, FN-HBD itself produces a modest effect on cell adhesion in the absence of central cell-binding domain (CCBD), FN-HBD significantly enhances cell adhesion and spreading activities by a cooperative mechanism of CCBD in MG63 cells (P < 0.05).