Self-assembled diphenylalanine nanowires for cellular studies and sensor applications

In this paper we present a series of experiments showing that vertical self-assembled diphenylalanine peptide nanowires (PNWs) are a suitable candidate material for cellular biosensing. We grew HeLa and PC12 cells onto PNW modified gold surfaces and observed no hindrance of cell growth caused by the...

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Bibliographic Details
Published inJournal of nanoscience and nanotechnology Vol. 12; no. 4; p. 3077
Main Authors Sasso, Luigi, Vedarethinam, Indumathi, Emnéus, Jenny, Svendsen, Winnie E, Castillo-León, Jaime
Format Journal Article
LanguageEnglish
Published United States 01.04.2012
Subjects
Animals
Biosensing Techniques
Cell Division
Electrodes
Gold
HeLa Cells
Humans
Microscopy, Electron, Scanning
Nanowires
PC12 Cells
Phenylalanine - analogs & derivatives
Phenylalanine - chemistry
Rats
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Summary:In this paper we present a series of experiments showing that vertical self-assembled diphenylalanine peptide nanowires (PNWs) are a suitable candidate material for cellular biosensing. We grew HeLa and PC12 cells onto PNW modified gold surfaces and observed no hindrance of cell growth caused by the peptide nanostructures; furthermore we studied the properties of PNWs by investigating their influence on the electrochemical behavior of gold electrodes. The PNWs were functionalized with polypyrrole (PPy) by chemical polymerization, therefore creating conducting peptide/polymer nanowire structures vertically attached to a metal electrode. The electroactivity of such structures was characterized by cyclic voltammetry. The PNW/PPy modified electrodes were finally used as amperometric dopamine sensors, yielding a detection limit of 3,1 microM.
ISSN:1533-4880
1533-4899
DOI:10.1166/jnn.2012.4534